Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-911 Aurora C Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human Aurora C amino acids 35-end & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end. For use in Kinase Assays.

Aurora C Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Kinase Assay UniProt Number: Q9UQB9
14-911
10 µg  Also available in 250µg size as catalog # 14-911M - Call for pricing and availability.
Purchase on Sigma-Aldrich

Overview

Key Spec Table

Key ApplicationsEntrez Gene NumberSpeciesUni Prot Number
KANP_001015878 Human Q9UQB9
Description
Catalogue Number14-911
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAurora C Protein, active, 10 µg
OverviewN-terminal 6His-tagged, recombinant, human Aurora C amino acids 35-end and N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end.
Combined purity 94% by SDS-PAGE and Coomassie blue staining. Aurora C MW = 36kDa, INCENP MW = 38kDa.
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal 6His-tagged, recombinant, human Aurora C amino acids 35-end & N-terminal GST-tagged, recombinant, human INCENP, amino acids 821-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceCo-expressed by baculovirus in Sf21 insect cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in
organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
Gene Symbol
  • AIK32 3
  • ARK-3
  • STK13
  • ARK3
  • AurC
Purification MethodNi2+/NTA agarose
UniProt Number
UniProt SummaryFunction: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Cofactor: Magnesium.
Enzyme regulation: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-172 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.
Subunit structure: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits.
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Molecular WeightAurora C MW = 36kDa, INCENP MW = 38kDa.
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of substrate peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250µg size as catalog # 14-911M - Call for pricing and availability.
Global Trade Item Number
Catalogue Number GTIN
14-911 04053252290879