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ABF192 Anti-phospho-MDA-5 (Ser104)

Anti-phospho-MDA-5 (Ser104), Cat. No. ABF192 is a rabbit polyclonal antibody that detects MDA-5 phosphorylated on Ser104 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-phospho-MDA-5 (Ser104) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: IFIH1, MDA5, RH116 UniProt Number: Q9BYX4
ABF192
200 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, PIARbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF192
DescriptionAnti-phospho-MDA-5 (Ser104)
Alternate Names
  • Interferon-induced helicase C domain-containing protein 1
  • CADM-140 autoantigen
  • Helicase with 2 CARD domains
  • Helicard
  • Interferon-induced with helicase C domain protein 1
  • Melanoma differentiation-associated protein 5
  • MDA-5
  • Murabutide down-regulated protein
  • RIG-I-like receptor 2
  • RLR-2
  • RNA helicase-DEAD box protein 116
Background InformationInterferon-induced helicase C domain-containing protein 1 (UniProt: Q9BYX4; also known as EC: 3.6.4.13, Clinically amyopathic dermatomyositis autoantigen 140 kDa, CADM-140 autoantigen, Helicase with 2 CARD domains, Helicard, Interferon-induced with helicase C domain protein 1, Melanoma differentiation-associated protein 5, MDA-5, Murabutide down-regulated protein, RIG-I-like receptor 2, RLR-2, RNA helicase-DEAD box protein 116) is encoded by the IFIH1 (also known as MDA5, RH116) gene (Gene ID: 64135) in human. MDA-5 is an innate immune receptor that acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and pro-inflammatory cytokines. MDA-5 is widely expressed, but at low levels. Expression is detected at slightly higher levels in placenta, pancreas and spleen. Its ligands include mRNA lacking 2'-O-methylation at their 5' cap and long-dsRNA. In the absence of ligands it is monomeric and homodimerizes in the presence of dsRNA ligands. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1), which activates the IKK-related kinases TBK1 and IKBKE that phosphorylate interferon regulatory factors IRF3 and IRF7. These factors activate transcription of antiviral immunological genes, including interferons alpha and beta. MDA-5 also plays an important role in enhancing natural killer cell function and is believed to be involved in growth inhibition and apoptosis in several tumor cell lines. MDA-5 contains 2 CARD domains; 1 helicase ATP-binding domain, 1 helicase C-terminal domain, and 1 RLR C-terminal regulatory domain.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-phospho-MDA-5 (Ser104), Cat. No. ABF192 is a rabbit polyclonal antibody that detects MDA-5 phosphorylated on Ser104 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
Key Applications
  • Western Blotting
  • Peptide Inhibition Assay
Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot detected phospho-MDA-5 (Ser104) in Raji cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids surrounding Ser104 from the N-terminal region of human MDA-5.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects human MDA-5 protein phosphorylated on Ser104.
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • IFIH1
  • MDA5
  • RH116
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~117 kDa observed; 116.69 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected phospho-MDA-5 (Ser104) in 10 µg of Daudi cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABF192 04054839316005