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ABS271 Anti-phospho-AS160 Antibody (Thr642)

Anti-phospho-AS160 Antibody (Thr642) is an antibody against phospho-AS160 (Thr642) for use in Western Blotting.

Anti-phospho-AS160 Antibody (Thr642) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Signaling Neuroscience Gene Symbol: TBC1D4, AS160, KIAA0603 UniProt Number: O60343
ABS271
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS271
DescriptionAnti-phospho-AS160 Antibody (Thr642)
Alternate Names
  • phospho-AS160 (Thr642)
  • TBC1 domain family member 4
  • Akt substrate of 160 kDa
  • AS160
Background InformationAS160 is a widely expressed RAB-GTPase activating protein which plays a role in insulin action. AS160 is phosphorylated by AKT, AMPK, RSK, and SGK1. Upon phosphorylation, AS160 changes its location from microsomes to the cytosol upon insulin treatment. AS160 acts as key link between insulin signaling and GLUT4 trafficking of glucose. Phosphorylation of AS160 is essential for AS160 action and AS160 has multiple phosphorylation sites. Phosphorylation of AS160 on both AS160Thr642 and AS160Ser588 is important for insulin-stimulated glucose transport. Phosphorylation of these sites is predicted to inhibit AS160's activation of Rab-GTPase proteins and favor greater exocytosis of GLUT4 vesicles, leading ultimately to greater glucose transport. Research indicates that impairment of phosphorylation of AS160 at Thr642 is associated with various aspects of type 2 diabetes.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-phospho-AS160 Antibody (Thr642) is an antibody against phospho-AS160 (Thr642) for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:200 dilution from a representative lot detected phospho-AS160 (Thr642) in 10 µg of Insulin treated NIH/3T3 cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phospho-AS160 (Thr642).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TBC1D4
  • AS160
  • KIAA0603
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~160 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in IGF-1 treated HEK293 cell lysate.

Western Blotting Analysis: A 1:200 dilution of this antibody detected phospho-AS160 (Thr642) in 10 µg of IGF-1 treated HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABS271 04055977136388