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ABE1304 Anti-monomethyl Histone H3 (Lys4) Antibody

Anti-monomethyl Histone H3 (Lys4) is an antibody against monomethyl Histone H3 (Lys4) for use in WB, IP, IH, Dot Blot.

Anti-monomethyl Histone H3 (Lys4) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ
 UniProt Number: P68431
ABE1304
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IP, IHC, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1304
DescriptionAnti-monomethyl Histone H3 (Lys4) Antibody
Alternate Names
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/I
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l
Background InformationHistones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histone modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Histone H3 is acetylated on Lys14 by Gcn5, and histone H3 phosphorylation at Ser10 facilitates acetylation of Lys14 by Gcn5 in vitro. Moreover, Lys14 is involved in chromatin remodeling, and RSC4 (part of the RSC chromatin remodeling complex) contains essential tandem bromodomains that require H3 Lys14 acetylation for gene activation.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-monomethyl Histone H3 (Lys4) is an antibody against monomethyl Histone H3 (Lys4) for use in WB, IP, IH, Dot Blot.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry
  • Dot Blot
Application NotesDot Blot Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in an Absurance™ Histone H3 Antibody Specificity Array (Cat. No. 16-667) and an Absurance™ Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665).
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl Histone H3 (Lys4) in 500 µg of HeLa acid extract.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H3 (Lys4) in human and rat testis tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human monomethyl Histone H3 (Lys4).
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST1H3A
  • H3FA
  • HIST1H3B
  • H3FL
  • HIST1H3C
  • H3FC
  • HIST1H3D
  • H3FB
  • HIST1H3E
  • H3FD
  • HIST1H3F
  • H3FI
  • HIST1H3G
  • H3FH
  • HIST1H3H
  • H3FK
  • HIST1H3I
  • H3FF
  • HIST1H3J
  • H3FJ
Modifications
  • Methylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected monomethyl Histone H3 (Lys4) in 10 µg of HeLa acid extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1304 04055977112597