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OP24 Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2)

This Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of c-K-Ras (Ab-1).

Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP24
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP24-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes c-K-Ras and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Cross-reacts with c-H-Ras and c-N-Ras.
Catalogue NumberOP24
Brand Family Calbiochem®
References
ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sirdransky, D., et al. 1992. Science 256, 102.
Shaw, P., et al. 1991. Oncogene 6, 2121.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlSW480 or Y1 cells or normal skin tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (10 µg/ml)
Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
Immunoprecipitation (see comments)
Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
Application CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, rat K-Ras
ImmunogenRat
Epitopewithin amino acids 54-189
Clone234-4.2
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP24-100UG 04055977208368

Documentation

Anti-c-K-Ras (Ab-1) Mouse mAb (234-4.2) Certificates of Analysis

TitleLot Number
OP24

References

Reference overview
Mizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sirdransky, D., et al. 1992. Science 256, 102.
Shaw, P., et al. 1991. Oncogene 6, 2121.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-June-2009 JSW
ApplicationFrozen Sections (10 µg/ml)
Immunoblotting (5 µg/ml with 1% gelatin, see comments and application references)
Immunoprecipitation (see comments)
Paraffin Sections (10 µg/ml, saponin or pepsin pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Ag8.653 mouse myeloma cells. Recognizes the ~21 kDa c-K-Ras and v-K-Ras proteins. Also recognizes c-H-Ras and c-N-Ras.
BackgroundThe human ras gene family consists of three identified members, H, K and N-ras, encoding proteins of 188-189 amino acids and 21,000 (p21) molecular weight. Human H- and K-ras are the homologues of v-H- and v-K-ras sequences originally isolated from Harvey and Kirsten strains of rat sarcoma viruses. Normal human cellular ras genes can be activated to oncogenes by mutations occurring in codons 12, 13 and 61; such mutated, activated and transforming ras genes have been identified and isolated from human tumors and cultured tumor cells. Although the expression patterns of Ras proteins in normal human tissues are known, similar information for activated ras oncogene encoded p21s and their relevance to human disease diagnosis and prognosis is still emerging. K-ras mutations have been identified in several cancers to date including lung and colorectal tumors.
HostMouse
Immunogen speciesRat
Immunogenrecombinant, rat K-Ras
Epitopewithin amino acids 54-189
Clone234-4.2
IsotypeIgG2a
Specieshuman, mouse, rat
Positive controlSW480 or Y1 cells or normal skin tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsThis antibody reacts with c-K-Ras and v-K-Ras, and cross-reacts with c-H-Ras or c-N- Ras by immunoblotting. The level of expression of p21 Ras is variable in different tissues. For this reason we recommend a sample concentration step prior to immunoblotting (see application references). For immunoprecipitation, use 5 µg Cat. No. OP24 per sample with 45 µl protein A agarose. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesMizuuchi, H. et al. 1992. Cancer Res. 2, 2777.
Rodenhuis, S. et al. 1992. Cancer Res. (Suppl) 52, 2665s.
Sirdransky, D., et al. 1992. Science 256, 102.
Shaw, P., et al. 1991. Oncogene 6, 2121.
Bos, J.L. 1989. Cancer Res. 49, 4682.
Furth, M.E., et al. 1987. Oncogene 1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA 81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112.
Taparowsky, E., et al. 1983. Cell 34, 581.
Ellis, R.W., et al. 1981. Nature 292, 506.
Shih, T.Y., et al. 1980. Nature 287, 686.
Application referencesImmunoblotting Palejwala, S. and Goldsmith, L.T. 1991. BioTechniques 11, 606.