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ABE269 Anti-acetyl-Histone H2B (Lys24) Antibody

Use Anti-acetyl-Histone H2B (Lys24) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP, DB to detect acetyl-Histone H2B (Lys24) also known as H2BK24Ac, Histone H2B (acetyl K24).

Anti-acetyl-Histone H2B (Lys24) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H2BB UniProt Number: Q8N257
ABE269
200 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IP, DBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE269
DescriptionAnti-acetyl-Histone H2B (Lys24) Antibody
Alternate Names
  • Histone H2B type 3-B
  • H2B type 12
Background InformationChanges in chromatin structure play a large role in the regulation of transcription in eukaryotes (1). The nucleosome is the primary building block of chromatin, and is made up of four core histone proteins (H2A, H2B, H3 and H4) (2). Acetylation of core histones regulates gene expression (2). Histone H2B is primarily acetylated at lysines 5, 12, 15, and 20 (3). Ubiquitylation of Histone H2B at lysine 123 by Rad6 has been implicated in transcriptional activation and lysine methylation on histone H3 (4). Histone H2B phosphorylation at Ser10 (for yeast) and Ser14 (for mammalian cells) has been linked to regulation of apoptosis and meiosis (5).
References
Product Information
FormatAffinity Purified
Control
  • Recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationUse Anti-acetyl-Histone H2B (Lys24) Antibody (Rabbit Polyclonal Antibody) validated in WB, IP, DB to detect acetyl-Histone H2B (Lys24) also known as H2BK24Ac, Histone H2B (acetyl K24).
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Dot Blot
Application NotesImmunoprecipitation Analysis: 10 μg from a representative lot immunoprecipitated acetyl-Histone H2B (Lys24) from HeLa acid extract.

Dot Blot Specificity Analysis: Unmodified and various modified Histone peptides (see Table) were probed with Anti-acetyl-Histone H2B (Lys24) (1:500 dilution).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human Histone H2B acetylated at Lys24.
EpitopeAcetylated Lys24
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes Histone H2B acetylated at Lys24.
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HIST3H2BB
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts.

Western Blot Analysis: A 1:500 dilution of this antibody detected Histone H2B on 10 µg of recombinant Histone H2B protein, and untreated and sodium butyrate treated HeLa acid extracts.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE269 04053252691911

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies