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MABF2347-100UG Anti-VSV Matrix Protein M Antibody, clone 23H12

MABF2347-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2347-100UG
DescriptionAnti-VSV Matrix Protein M Antibody, clone 23H12
Alternate Names
  • Matrix Protein Vesicular stomatitis virus
  • VSIV Matrix protein
Background InformationMatrix protein from Vesicular stomatitis virus (VSV) (UniProt: P03519; also known as Protein M) is encoded by the M gene (Gene ID: 1489833) in Vesicular stomatitis Indiana virus. VSV belongs to the Rhabdoviridae family and two distinct serotypes of this virus have been described the New Jersey and Indiana type. It is an enveloped, negative-sense RNA virus that mainly infects a variety domestic animals and causes vesiculation, ulceration, and erosion of the oral and nasal mucosa and epithelial surface of the tongue. The genomic structure of this virus is a single strand of negative-sense RNA that is composed of five genes known as N, P, M, G, and L that represent the nucleocapsid protein, phosphoprotein, matrix protein, glycoprotein, and the large protein, which is a component of the viral RNA polymerase. The matrix protein (M) is a homomultimeric protein that plays a major role in assembly and budding of virion, by recruiting cellular partners of the ESCRT complexes. It is also responsible for most of the cytopathic effects observed in infected cells. M protein is reported to interfere with the transport of cellular mRNAs from the nucleus to the cytoplasm and modulate the redistribution of the splicing factor. It can shut off cellular protein expression by inhibiting mRNA nuclear export through direct interaction with host RAE1-NUP98 complex. This shutoff inhibits interferon signaling and establishes an antiviral state in virus infected cells. M protein contains a dynamin binding motif (aa 2-4); a PPXY motif (aa 24-27), and a PTAP/PSAP motif (aa 37-40). The latter two motifs are reported to contribute to virus egress from infected cells. Interaction between these motifs and ESCRT pathway components are also essential for viral assembly. (Ref.: Redondo, N., et al. (2015). PLoS ONE 10(6): e0131137; D'agostino, PM., et al. (2009). Viral Immunol. 22(6): 353-369).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-VSV Matrix Protein M, clone 23H12, Cat. No. MABF2347, is a mouse monoclonal antibody that detects VSV-Matrix protein and is tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected VSV-Matrix protein in Western Blotting applications (Brinkmann, C., et. al. (2017). PLoS One. 12(12):e0189073; Redondo, N., et. al. (2015). PLoS One. 10(6): e0131137; Rogalin, H.B., et. al. (2016). J Virol. 90(22): 10321-10328).

Immunocytochemistry Analysis: A representative lot detected VSV-Matrix protein in Immunocytochemistry applications (Whitt, M.A., et. al. (2010). J Virol Methods. 169(2): 365-74).

Immunofluorescence Analysis: A representative lot detected VSV-Matrix protein in Immunofluorescence applications (Redondo, N., et. al. (2015). PLoS One. 10(6): e0131137).

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected VSV-Matrix protein in BHK cell lysates infected with VSV-Orsay (Courtesy of Shelby Puckett (Lyles Lab), Wake Forest School of Medicine, North Carolina USA).
Biological Information
ImmunogenVesicular stomatitis virus, Indiana serotype.
Clone23H12
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 23H12 is a mouse monoclonal antibody that detects Vesicular stomatitis virus matrix protein M in virus infected cells.
IsotypeIgG2aκ
Species Reactivity
  • Virus
Species Reactivity NoteVirus (Vesicular stomatitis).
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • M
Purification MethodProtein G purified
UniProt Number
Molecular Weight26.09 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2ak.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2347-100UG 04061841440741

Documentation

Anti-VSV Matrix Protein M Antibody, clone 23H12 SDS

Title

Safety Data Sheet (SDS) 

Anti-VSV Matrix Protein M Antibody, clone 23H12 Certificates of Analysis

TitleLot Number
Anti-VSV Matrix Protein M, clone 23H12 - 3499338 3499338
Anti-VSV Matrix Protein M, clone 23H12 - 3519321 3519321
Anti-VSV Matrix Protein M, clone 23H12 - 3558979 3558979
Anti-VSV Matrix Protein M, clone 23H12 - 3560029 3560029
Anti-VSV Matrix Protein M, clone 23H12 - 3590121 3590121
Anti-VSV Matrix Protein M, clone 23H12 - 3594296 3594296
Anti-VSV Matrix Protein M, clone 23H12 - 3597269 3597269
Anti-VSV Matrix Protein M, clone 23H12 - 3600658 3600658
Anti-VSV Matrix Protein M, clone 23H12 - 3601706 3601706
Anti-VSV Matrix Protein M, clone 23H12 - 3603632 3603632

References

Reference overviewPub Med ID
The glycoprotein of vesicular stomatitis virus promotes release of virus-like particles from tetherin-positive cells.
Brinkmann, C; Hoffmann, M; Lübke, A; Nehlmeier, I; Krämer-Kühl, A; Winkler, M; Pöhlmann, S
PLoS One  12  e0189073  2017

Show Abstract
29216247 29216247
Characterization of Vesicular Stomatitis Virus Pseudotypes Bearing Essential Entry Glycoproteins gB, gD, gH, and gL of Herpes Simplex Virus 1.
Rogalin, HB; Heldwein, EE
J Virol  90  10321-10328  2016

Show Abstract
27605677 27605677
Impact of Vesicular Stomatitis Virus M Proteins on Different Cellular Functions.
Redondo, N; Madan, V; Alvarez, E; Carrasco, L
PLoS One  10  e0131137  2015

Show Abstract
26091335 26091335
Generation of VSV pseudotypes using recombinant ΔG-VSV for studies on virus entry, identification of entry inhibitors, and immune responses to vaccines.
Whitt, MA
J Virol Methods  169  365-74  2010

Show Abstract
20709108 20709108