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CP06 Anti-α-Tubulin Mouse mAb (DM1A)

This Anti-α-Tubulin Mouse mAb (DM1A) is validated for use in Immunoblotting, Immunofluorescence for the detection of α-Tubulin.

Anti-α-Tubulin Mouse mAb (DM1A) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CP06
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Ch, Gr, H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
CP06-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~60 kDa tubulin protein found in all eukaryotic cells. May be used as a positive control.
Catalogue NumberCP06
Brand Family Calbiochem®
References
ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controltrp E (Ab-1)
Positive controlAny cell line
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesImmunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)
Immunofluorescence (5 µg/ml)
Application CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogennative chick brain microtubules
ImmunogenChicken
CloneDM1A
HostMouse
IsotypeIgG₁
Species Reactivity
  • Chicken
  • Gerbil
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CP06-100UG 04055977220933

Documentation

Anti-α-Tubulin Mouse mAb (DM1A) SDS

Title

Safety Data Sheet (SDS) 

Anti-α-Tubulin Mouse mAb (DM1A) Certificates of Analysis

TitleLot Number
CP06

References

Reference overview
Blose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.

Citations

Title
  • Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058.
  • Zhonghua Gao, Yufang Shao and Xuejun Jiang. (2005) Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis. Journal of Biological Chemistry 280, 38271-38275.
  • Annie Huang, et al. (2005) Identification of a novel c-myc protein interactor, JPO2, with transforming activity in medulloblastoma cells. Cancer Research 65, 5607-5619.
  • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision30-November-2007 JSW
    ApplicationImmunoblotting (20-100 ng/ml, colorimetric or chemiluminescence; see application references)
    Immunofluorescence (5 µg/ml)
    DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c57 mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~60 kDa tubulin protein.
    BackgroundCytoskeletal proteins are found in highly organized arrays within the cytoplasm of higher eukaryotic cells. These proteins are intimately involved in functions such as cell and intracellular organelle movement, maintenance of cell shape, and endocytosis. The cytoskeleton of most eukaryotic cells is comprised of at least three fibrous systems each with accessory proteins: the microfilaments, composed of actin; the microfibrils, composed of tubulin; and the intermediate filaments composed of vimentin. Monoclonal antibodies have been used to study the components of the cytoskeleton and their intracellular location, changes in the expression of these elements during differentiation, and the function of the cytoskeleton in living cells by microinjection of the antibodies.
    HostMouse
    Immunogen speciesChicken
    Immunogennative chick brain microtubules
    CloneDM1A
    IsotypeIgG₁
    Specieschicken, gerbil, human, mouse, rat
    Positive controlAny cell line
    Negative controltrp E (Ab-1)
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsTubulin is found in all cells and is used as a positive control for immunofluorescence or immunoblotting. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesBlose, S.H., et al. 1984. J. Cell. Biol. 98, 847.
    Lin, J.J.C., et al. 1984. In: Monoclonal antibodies and functional cell lines. Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds. Plenum Publishing Corp.
    Lin, J.J.C. 1982. Cold Spring Harbor Symp. on Quant. Biol. 46, 769.
    Goldman, R.D., et al. 1979. Ann. Rev. Physiol. 41, 701.
    Goldman, R., Pollard, T. and Rosenbaum, J. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
    Citation
  • Saumen Pal, et al. (2006) An antiangiogenic neurokinin-B/thromboxane A2 regulatory axis. Journal of Cell Biology 174, 1047-1058.
  • Zhonghua Gao, Yufang Shao and Xuejun Jiang. (2005) Essential roles of the Bcl-2 family of proteins in caspase-2-induced apoptosis. Journal of Biological Chemistry 280, 38271-38275.
  • Annie Huang, et al. (2005) Identification of a novel c-myc protein interactor, JPO2, with transforming activity in medulloblastoma cells. Cancer Research 65, 5607-5619.
  • Thomas Simmen, et al. (2005) PACS-2 controls endoplasmic reticulum-mitochondria communication and Bid-mediated apoptosis. European Molecular Biology Organization Journal 24, 717-729.
    		•
    Application referencesImmunoblotting Gagarin, D., et al. 2005. J. Mol. Cell. Card. 39, 453. Yang, Z., et al. 2007. J. Vasc. Res. 44, 483.