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AB3245-I Anti-TopBP1

Anti-TopBP1 Antibody, Cat. No. AB3245-I, is a highly specific rabbit polyclonal antibody that targets DNA topoisomerase 2-binding protein 1 (TopBP1) and has been tested in Immunoprecipitation and Western Blotting.

Anti-TopBP1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: TOPBP1, KIAA0259 UniProt Number: Q92547
AB3245-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3245-I
DescriptionAnti-TopBP1
Alternate Names
  • DNA topoisomerase 2-binding protein 1
  • DNA topoisomerase II-beta-binding protein 1
  • DNA topoisomerase II-binding protein 1
  • TopBP1
Background InformationDNA topoisomerase 2-binding protein 1 (UniProt Q92547; also known as DNA topoisomerase II-beta-binding protein 1, DNA topoisomerase II-binding protein 1, TopBP1) is encoded by the TOPBP1 (also known as KIAA0259) gene (Gene ID 11073) in human. Timely DNA replication and accurate chromosome segregation is critical for maintaining genome integrity to avoid cancer and other genetic diseases. TopBP1 plays an important role in mediating DNA processing during mitosis to reduce transmission of DNA damage toG1 daughter cells. Unlike the translesion synthesis scaffold factor Rev1 or K164-ubiquitylated PCNA that serve to recruit translesion polymerases to stalled forks, TopBP1 forms foci upon mitotic entry, marking sites of and promoting unscheduled DNA synthesis at underreplicated regions independent of Rev1 and PCNA. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induces formation of 53BP1 NBs in the next cell cycle. Human TopBP1 contains eight BRCA1 C Terminus (BRCT) domains (a.a. 101-189, 195-284, 354-444, 548-633, 641-738, 900-991, 1259-1351, 1389-1486) and two nuclear localization signal (NLS) motifs (a.a. 852-858, 1517-1520). TopBP1 BRCT1/2-mediated interaction with phosphorylated Rad9 is essential for TopBP1-dependent ATR (ataxia telangiectasia and Rad3-related) activation, while TopBP1 BRCT6 interacts with the phosphorylated transcription factor, E2F1 to repress E2F1 activity in response to DNA damage. BRCT6 is also targeted for poly(ADP-ribosyl)ation by poly(ADP-ribose) polymerase 1 (PARP-1). The innate immune regulator STAT-5 is activated by human papillomavirus (HPV) proteins in HIV-infected cells, resulting in upregulated TopBP1 transcription and ATR pathway activation required for HPV genome amplification.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Quality LevelMQ100
Applications
ApplicationAnti-TopBP1 Antibody, Cat. No. AB3245-I, is a highly specific rabbit polyclonal antibody that targets DNA topoisomerase 2-binding protein 1 (TopBP1) and has been tested in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
Application NotesImmunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated TopBP1 from 500 µg of HeLa cell lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a squence within the region between BRCT domain 6 and 7 of human TopBP1.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody targets human TopBP1 region between the sixth and seventh BRCA1 C-terminus (BRCT) domain.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • TOPBP1
  • KIAA0259
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~180 kDa observed. 170.7 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TopBP1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB3245-I 04054839057168