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MABN2320-100UG Anti-TREM-2 Antibody, C-terminal Antibody, clone 14D1

MABN2320-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABN2320-100UG
DescriptionAnti-TREM-2 Antibody, C-terminal Antibody, clone 14D1
Alternate Names
  • Triggering receptor expressed on myeloid cells 2
  • Triggering receptor expressed on monocytes 2
Background InformationTriggering receptor expressed on myeloid cells 2 (UniProt: Q99NH8; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the Trem2 (also known as Trem2a, Trem2b, Trem2c) gene (Gene ID: 83433) in murine species. TREM-2 is a single-pass type I monomeric membrane protein that forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. TREM-2 is expressed at higher levels in the central nervous system (CNS), heart, and lung. At lower levels it is also expressed in lymph nodes and in non-lymphoid tissues such as kidney, liver and testis. In the CNS it is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. It plays a role in chronic inflammation and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. TREM-2 forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Apolipoprotein E (ApoE) is reported to function as a high-affinity ligand for TREM-2. ApoE binding is shown to enhance the phagocytosis of apoptotic N2a cells by primary microglia cells. Greatly reduced ApoE binding is seen with Alzheimer s disease-associated TREM2 R47H mutant. TREM2 is synthesized with a signal peptide (aa 1-18) that is cleaved off to generate the mature form with a large extracellular region (aa 19-171) that contains the V-type Ig-like domain, a single-transmembrane domain (aa 172-192), and a cytoplasmic tail (aa 193-227). The short cytoplasmic tail does not contain any signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction. (Ref.: Kleinberger, G., et al. (2017). EMBO J. 36(13); 1837-1853).
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Trem2, C-terminal, clone 14D1, Cat. No. MABN2320, is a highly specific rat monoclonal antibody, that targets Triggering receptor expressed on myeloid cells 2 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:25 dilution from a representative lot detected TREM-2 in mouse cerebral cortex and olfactory lobe tissue sections.

Western Blotting Analysis: A representative lot detected TREM-2 in Western Blotting applications (Kleinberger, G., et. al. (2017). EMBO J. 36(13):1837-1853).
Biological Information
ImmunogenA linear peptide corresponding to 20 amino acids from the C-terminal region of murine Triggering receptor expressed on myeloid cells 2 (TREM-2).
Clone14D1
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone 14D1 is a rat monoclonal antibody that specifically detects murine Triggering receptor expressed on myeloid cells 2 (TREM-2). It targets an epitope with in 20 amino acids from the C-terminal region.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Trem2
  • Trem2a
  • Trem2c
Purification MethodProtein G purified
UniProt Number
Molecular Weight24.53 kDa calculated.
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse hindbrain tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected TREM-2 in mouse hindbrain tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
MABN2320-100UG 04054839990526