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MABE426-I-25UL Anti-Sirt1(Sir2) Antibody, clone 3-10 Antibody, rabbit monoclonal

Anti-Sirt1(Sir2), clone 3-10, Cat. No. MABE426-I, is a highly specific rabbit monoclonal antibody that targets NAD-dependent protein deacetylase sirtuin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABE426-I-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE426-I-25UL
DescriptionAnti-Sirt1(Sir2) Antibody, clone 3-10 Antibody, rabbit monoclonal
Alternate Names
  • NAD-dependent protein deacetylase sirtuin-1
  • Regulatory protein SIR2 homolog 1
  • SIR2-like protein 1
  • SIR2alpha
  • Sir2
  • mSIR2a
Background InformationNAD-dependent protein deacetylase sirtuin-1 (UniProt: Q923E4; also known as Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SIR2alpha, Sir2, mSIR2a, Sirt-1) is encoded by the Sirt1 (also known as Sir2l1) gene (Gene ID: 93759) in murine species. Sirt1 is a NAD-dependent protein deacetylase that is widely expressed and links transcriptional regulation directly to intracellular energetics. In liver, Sirt1 is expressed in a circadian manner with maximal and minimal levels reaching at around Zeitgeber time (ZT) 16 and ZT4, respectively and its deacetylase activity is also regulated in a circadian manner that peaks at ZT15. It participates in the coordination of several cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis, and autophagy. It is shown to modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. It deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression in both positive and negative manner. Sirt1 serves as a sensor of the cytosolic ratio of NAD+/NADH, which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Sirt1 has four nucleotide (NAD)-binding regions and four zinc binding sites. It is reported to shuttle between nucleus and cytoplasm. Sir1 is proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield a catalytic inactive but stable 75 kDa fragment (aa 2-525). Sirt1 can be phosphorylated by multiple protein kinases. It is phosphorylated by STK4/MST1 that results in inhibition of SIRT1-mediated p53/TP53 deacetylation and phosphorylation by MAPK8/JNK1 at serine 46 and threonine 522 is reported to increase its nuclear localization and enzymatic activity. Sirt1 phosphorylation at threonine 522 by DYRK1A and DYRK3 activates its deacetylase activity and can promote cell survival.
References
Product Information
FormatUnpurified
PresentationRabbit monoclonal antibody in cell culture supernatant with 0.05% sodium azide.
Applications
ApplicationAnti-Sirt1(Sir2), clone 3-10, Cat. No. MABE426-I, is a highly specific rabbit monoclonal antibody that targets NAD-dependent protein deacetylase sirtuin-1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Sirt1(Sir2) in mouse cerebral cortex tissue sections.
Biological Information
ImmunogenHis-tagged full-length recombinant mouse NAD-dependent protein deacetylase sirtuin-1 (Sirt1).
Clone3-10
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityClone 3-10 specifically detects murine Sirt1.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Sirt1
  • Sir2l1
Purification MethodUnpurified
UniProt Number
Molecular Weight~110 kDa observed; 80.37 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Sirt1(Sir2) in NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE426-I-25UL 04054839646683

Documentation

Anti-Sirt1(Sir2) Antibody, clone 3-10 Antibody, rabbit monoclonal SDS

Title

Safety Data Sheet (SDS)