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PC177L Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb

This Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb is validated for use in Frozen Sections, Immunofluorescence for the detection of Serotonin (5-HT) Transporter (602-622).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC177L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
M, RRbPolyclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
PC177L-100UL Plastic ampoule 100 ul
Description
OverviewRecognizes the serotonin (5-HT) transporter in hypothalamus raphe nuclei and spinal cord.
Catalogue NumberPC177L
Brand Family Calbiochem®
Application Data
Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
References
ReferencesZhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Product Information
FormLyophilized
FormulationUndiluted serum.
Positive controlRat naphe nuclei, hypothalamus, cortex, or spinal cord
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesFrozen Sections, Mouse Cross-Reactivity Salichon N., et al. 2001. J. Neuerosci. 21, 884.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesFrozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)
Application CommentsAntibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC177L-100UL 04055977209525

Documentation

Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb Certificates of Analysis

TitleLot Number
PC177L

References

Reference overview
Zhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision05-June-2008 JSW
ApplicationFrozen Sections (1:10,000-1:15,000; see application references)
Immunoblotting (see comments)
Immunofluorescence (1:200-1:400)
Application Data
Detection of rat serotonin (5-HT) transporter by immunohistochemistry. Sample: Rat raphe nuclei, hypthalamus, cortex, and spinal cord. Primary antibody: Anti-Serotonin (5-HT) Transporter (602-622) Rabbit pAb (Cat. No. PC177L) (1:1000). Detection: fluorescence.
DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the serotonin (5-HT) transporter protein.
BackgroundThe serotonin (5-hydroxytryptamine; 5-HT) transporter (5-HTT) plays an important role in the re-uptake of serotonin from the nerve terminal to ultimately terminate the serotonin signal. The 5-HTT plays a critical role in the regulation of serotonin neurotransmission, which has been linked to mood and personality disorders, anxiety, eating disorders, and insomnia. The predicted 12 transmembrane serotonin transporter contains potential sites for phosphorylation and glycosylation. Because serotonin is involved in such a variety of biological processes, the 5-HTT has been a target for the development of antidepressants and psychostimulants. Blocking serotonin uptake at the synapse has been achieved using structurally related chemicals called Selective Serotonin Re-uptake Inhibitors (SSRI) which alleviate disorders such as depression and alcoholism. The 5-HTT is localized to the plasma membrane of neurons and glial cells, as well as blood platelets, placenta, and endothelial cells.
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide corresponding to amino acids 602-622 of rat 5-HT transporter
IsotypeIgG
Speciesmouse, rat
Positive controlRat naphe nuclei, hypothalamus, cortex, or spinal cord
FormLyophilized
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsAntibody specificity was examined in the rat cortex, hypothalamus, raphe nuclei, and spinal cord. Staining is completely eliminated by pretreatment of antibody with Serotonin (5-HT) Transporter Control Peptide (Cat. No. PP87) at a concentration of 5 µg/ml. Low levels of glutaraldehyde (0.1%-0.3%) may be used in conjunction with paraformaldehyde for tissue perfusion fixation. The specificity of this antibody was also determined by immunoblotting analysis using rat brain extracts of the cortex, hypothalamus, midbrain, and hindbrain, which specifically detected a single band. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with 100 µl of sterile distilled H₂O. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Further dilute with sterile PBS or Tris buffer to dilutions no higher than 1:10.
Toxicity Standard Handling
ReferencesZhou, F.C., et al. 1996. Mol. Brain Res. 43, 267.
Blakely, R.D., et al. 1994. J. Exp. Biol. 196, 263.
Coccaro, E.F. and Murphy, D.L. (Eds.) 1990. in Serotonin in Major Disorders. The Progress in Psychiatry Series, American Psychiatric Press, Washington, D.C.
Application referencesFrozen Sections, Mouse Cross-Reactivity Salichon N., et al. 2001. J. Neuerosci. 21, 884.