Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABE1013-25UG Anti-RRP8 Antibody, clone 5C7.1

Anti-RRP8, clone 5C7.1, Cat. No. MABE1013, is a highly specific mouse monoclonal antibody, that targets Ribosomal RNA-processing protein 8 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-RRP8 Antibody, clone 5C7.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: RRP8, KIAA0409, NML, hucep-1 UniProt Number: O43159
MABE1013-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ICCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1013-25UG
DescriptionAnti-RRP8 Antibody, clone 5C7.1
Alternate Names
  • Ribosomal RNA-processing protein 8
  • Cerebral protein 1
  • Nucleomethylin
Background InformationRibosomal RNA-processing protein 8 (UniProt: O43159; also known as Cerebral protein 1, Nucleomethylin) is encoded by the RRP8 (also known as KIAA0409, NML, hucep-1) gene (Gene ID: 23378) in human. Ribosome biosynthesis is one of the most energy-consuming cellular process, which adapts to changes in intracellular energy status. Ribosomal RNA-processing protein 8 is a novel nuclear protein that serves as an essential component of the energy-dependent nucleolar silencing) complex (eNoSC) that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation or upon elevation of NAD+/NADP+ ratio and activates SIRT1 that leads to deacetylation of histone H3 followed by dimethylation of H3 at Lysine 9 (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-RRP8, clone 5C7.1, Cat. No. MABE1013, is a highly specific mouse monoclonal antibody, that targets Ribosomal RNA-processing protein 8 and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RRP8 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Biological Information
ImmunogenGST-tagged recombinant fragment corresponding to 98 amino acids from the C-terminal half of human Ribosomal RNA-processing protein 8.
Clone5C7.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5C7.1 specifically detects Ribosomal RNA-processing protein 8. It targets an epitope with in the C-terminal half.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RRP8
  • KIAA0409
  • NML
  • hucep-1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~53 kDa observed; 50.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected RRP8 in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1013-25UG 04054839519543