Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

04-1569 Anti-RNA polymerase II subunit B1 Antibody, clone 4F8

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors, RNA Metabolism & Binding Proteins Gene Symbol: POLR2A, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 UniProt Number: P24928
04-1569
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HWB, ELISA, ChIPRPurifiedMonoclonal Antibody
Description
Catalogue Number04-1569
DescriptionAnti-RNA polymerase II subunit B1 Antibody, clone 4F8
Alternate Names
  • DNA-directed RNA polymerase II A
  • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA polymerase II subunit B1
  • RNA-directed RNA polymerase II subunit RPB1
  • polymerase (RNA) II (DNA directed) polypeptide A (220kD)
  • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
Background InformationRNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II complex. As a holoenzyme RNA polymerase II catalyzes transcription of eukaryotic DNA into RNA using the four ribonucleoside triphosphates as substrates. The RBP1 subunit, in combination with other polymerase subunits, forms a large central cleft that maintains contact between the active site of the enzyme, the DNA template, and the nascent RNA transcript. This subunit also contains a carboxy terminal domain (CTD) consisting of tandem heptapeptide repeats. In actively transcribing RNA polymerase ‘Ser-2’ and ‘Ser-5’ of the heptapeptide repeat are phosphorylated. Phosphorylation activates the RNA polymerase II beta subunit, allowing it to serve as an assembly platform for additional subunits that modulate initiation, elongation, termination and mRNA processing.
References
Product Information
FormatPurified
Control
  • NIH/3T3 cell lysates
PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RNA polymerase II subunit B1 Antibody, clone 4F8 is a Rat monoclonal antibody for detection of RNA polymerase II subunit B1 also known as DNA-directed RNA polymerase II A & has been validated in WB, ELISA.
Key Applications
  • Western Blotting
  • ELISA
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation Analysis: A representative lot was used by independent laboraotry in ChIP. (Chapman, R., et al. (2007). Science. 318(5857):1780 -1782.)
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to human RNA polymerase II subunit B1.
EpitopeUnknown
Clone4F8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificityThis antibody recognizes RNA polymerase II subunit B1.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteDemonstrated to react with mouse. Predicted to react with human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. [provided by RefSeq].
Gene Symbol
  • POLR2A
  • POLR2
  • POLRA
  • RPB1
  • RPBh1
  • RPO2
  • RPOL2
  • RpIILS
  • hRPB220
  • hsRPB1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. Acts as a RNA-dependent RNA polymerase when associated with small delta antigen of Hepatitis delta virus, acting both as a replicate and transcriptase for the viral RNA circular genome.

CATA;YTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

SUBUNIT STRUCTURE: Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. The phosphorylated C-terminal domain interacts with FNBP3 and SYNCRIP. Interacts with SAFB/SAFB1. Interacts with CCNL1 and MYO1C By similarity. Interacts with CCNL2 and SFRS19. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts with PAF1. Interacts (via C-terminus) with FTSJD2.

SUBCELLULAR LOCATION: Nucleus

PTM: The tandem 7 residues repeats in the C-terminal domain (CTD) can be highly phosphorylated. The phosphorylation activates Pol II. Phosphorylation occurs mainly at residues 'Ser-2' and 'Ser-5' of the heptapepdtide repeat. The phosphorylation state is believed to result from the balanced action of site-specific CTD kinases and phosphataes, and a "CTD code" that specifies the position of Pol II within the transcription cycle has been proposed.

MISCELLANEOUS: The binding of ribonucleoside triphosphate to the RNA polymerase II transcribing complex probably involves a two-step mechanism. The initial binding seems to occur at the entry (E) site and involves a magnesium ion temporarily coordinated by three conserved aspartate residues of the two largest RNA Pol II subunits. The ribonucleoside triphosphate is transferred by a rotation to the nucelotide addition (A) site for pairing with the template DNA. The catalytic A site involves three conserved aspartate residues of the RNA Pol II largest subunit which permanently coordinate a second magnesium ion.

SEQUENCE SIMILARITIES: Belongs to the RNA polymerase beta' chain family.
Molecular Weight~ 220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in NIH/3T3 cell lysates.

Western Blot Analysis: 1 µg/ml of this antibody detected RNA polymerase II subunit B1 on 10 µg of NIH/3T3 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1569 04053252552182

Documentation

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 SDS

Title

Safety Data Sheet (SDS) 

Anti-RNA polymerase II subunit B1 Antibody, clone 4F8 Certificates of Analysis

TitleLot Number
Anti-RNA polymerase II subunit B1, clone 4F8 2470766
Anti-RNA polymerase II subunit B1, clone 4F8 - 2446745 2446745
Anti-RNA polymerase II subunit B1, clone 4F8 - 2064323 2064323
Anti-RNA polymerase II subunit B1, clone 4F8 - 2293534 2293534
Anti-RNA polymerase II subunit B1, clone 4F8 - 3156839 3156839
Anti-RNA polymerase II subunit B1, clone 4F8 - 3557814 3557814
Anti-RNA polymerase II subunit B1, clone 4F8 - 3856224 3856224
Anti-RNA polymerase II subunit B1, clone 4F8 - 4086892 4086892
Anti-RNA polymerase II subunit B1, clone 4F8 - NG1873218 NG1873218
Anti-RNA polymerase II subunit B1, clone 4F8 - NG1946568 NG1946568
Show more

References

Reference overviewPub Med ID
Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7.
Chapman, Rob D, et al.
Science, 318: 1780-2 (2007)  2007

Show Abstract 		18079404 18079404

Related Products & Applications

Related Products

Catalogue Number Description
04-1570 Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
04-1570-I Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-7) Antibody, clone 4E12
04-1571 Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) Antibody, clone 3E10
04-1571-I Anti-RNA polymerase II subunit B1 (phospho CTD Ser-2), clone 3E10 (rat monoclonal).
04-1572 Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
04-1572-I Anti-RNA polymerase II subunit B1 (phospho-CTD Ser-5) Antibody, clone 3E8
05-623 Anti-RNA polymerase II Antibody, clone CTD4H8
05-623-AF488 Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 488 Conjugate
05-623-AF647 Anti-RNA polymerase II Antibody, clone CTD4H8, AlexaFluor® 647 Conjugate
05-623-Z Anti-RNA polymerase II Antibody, clone CTD4H8, Ascites Free
Show more

Categories

Life Science Research > Antibodies and Assays > Primary Antibodies