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04-1460 Anti-PRMT5 Antibody, clone 23C7

Use Anti-PRMT5 Antibody, clone 23C7 (mouse monoclonal antibody) validated in WB to detect PRMT5 also known as 72 kDa ICCln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog.

Anti-PRMT5 Antibody, clone 23C7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins, RNA Metabolism & Binding Proteins Gene Symbol: PRMT5, HRMT1L5, IBP72, SKB1Hs, JBP1, SKB1 UniProt Number: O14744
04-1460
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-1460
ReplacesMABE551
DescriptionAnti-PRMT5 Antibody, clone 23C7
Alternate Names
  • 72 kDa ICln-binding protein
  • HMT1 hnRNP methyltransferase-like 5
  • Jak-binding protein 1
  • SKB1 homolog
  • SKB1 homolog (S. pombe)
  • Shk1 kinase-binding protein 1 homolog
  • protein arginine methyltransferase 5
  • skb1 (S. pombe) homolog
Background InformationArginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.
References
Product Information
FormatPurified
Control
  • K562 cell lysate
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-PRMT5 Antibody, clone 23C7 (mouse monoclonal antibody) validated in WB to detect PRMT5 also known as 72 kDa ICCln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog.
Key Applications
  • Western Blotting
Biological Information
ImmunogenRecombinant protein corresponding to human PRMT5.
Clone23C7
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes PRMT5.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRMT5
  • HRMT1L5
  • IBP72
  • SKB1Hs
  • JBP1
  • SKB1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.

SIZE: 637 amino acids; 72684 Da

SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

SUBCELLULAR LOCATION: Cytoplasm.

TISSUE SPECIFICITY: Ubiquitous.

PTM: Disulfide bonds and non-covalent association mediate homooligomers formation.

SIMILARITY: Belongs to the protein arginine N-methyltransferase family.
Molecular Weight ~72 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in K562 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PRMT5 on 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
04-1460 04053252629136