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MABS818 Anti-PRKAA1, clone 2B7 Antibody

Detect PRKAA1 using this Anti-PRKAA1, clone 2B7 Antibody validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and ELISA.

Anti-PRKAA1, clone 2B7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PRKAA1, AMPK1 UniProt Number: Q13131
MABS818
100 µL  
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Overview

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IHC, ICC, FC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABS818
Replaces04-323
DescriptionAnti-PRKAA1, clone 2B7 Antibody
Alternate Names
  • 5'-AMP-activated protein kinase catalytic subunit alpha-1
  • AMPK subunit alpha-1
  • PRKAA1
Background Information5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1) belongs to the ser/thr protein kinase family. PRKAA1 is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Product Information
FormatAscites
PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationDetect PRKAA1 using this Anti-PRKAA1, clone 2B7 Antibody validated for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, Flow Cytometry and ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
  • Flow Cytometry
  • ELISA
Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in ovarian cancer and brain tissues.

Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKAA1 in Ntera-2 cells.

Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected PRKAA1 in PC-2 cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human PRKAA1 expressed in E. Coli.
Clone2B7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PRKAA1
  • AMPK1
Purification MethodUnpurified
UniProt Number
Molecular Weight~65 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKAA1 in Jurkat, HeLa, HepG2, MCF-7, Cos7, NIH/3T3, K562, HEK293, and PC-12 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MABS818 04055977172362