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MABN1801-25UG Anti-PMCA4b Antibody, clone JA3

Anti-PMCA4b, clone JA3, Cat. No. MABN1801, is a highly specific mouse monoclonal antibody that targets PMCA4b and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-PMCA4b Antibody, clone JA3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ATP2B4, ATP2B2, MXRA1 UniProt Number: P23634
MABN1801-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, ChpELISA, ICC, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1801-25UG
DescriptionAnti-PMCA4b Antibody, clone JA3
Alternate Names
  • Plasma membrane calcium-transporting ATPase 4
  • EC: 3.6.3.8
  • Matrix-remodeling-associated protein 1
  • Plasma membrane calcium ATPase isoform 4
  • Plasma membrane calcium pump isoform 4
Background InformationPlasma membrane calcium-transporting ATPase 4 (UniProt: P23634; also known as EC: 3.6.3.8, PMCA4, Matrix-remodeling-associated protein 1, Plasma membrane calcium ATPase isoform 4, Plasma membrane calcium pump isoform 4) is encoded by the ATP2B4 (also known as ATP2B2, MXRA1) gene (Gene ID: 493) in human. PMCA4 is a multi-pass membrane protein that acts as a calcium/calmodulin-regulated and magnesium-dependent enzyme and catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. PMCA4 contains 2 calmodulin binding domains known as Domain A (aa 1086-1103) and B (aa 1104-1113). PMCA4 is shown to be a regulator of neuronal nitric-oxide synthase (nNOS) and is suggested to play a structural role in tethering nNOS to a highly compartmentalized domain in the cardiac cell membrane. PMCA4b is also a major PMCA expressed in developing mammary tissue. PCMA4b form contains a PDZ domain that lies within the last 7 amino acids of the carboxyl terminus in mouse, which is absent in PCMAa tail. The kinetics of binding of Ca2+/CaM to PMCA4b is very slow, but remains active for longer periods when compared to PMCA4a. A significantly reduced migration of PCMA4b expressing in B-RAF mutant cells is linked to their reduced metastatic potential. (Ref.: Afronze, T., et al. (2014). J. Biol. Chem.289 (10), 7221-31); Mohanmed TM et al. (2011). J. Biol. Chem. 286(48):41520-29).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-PMCA4b, clone JA3, Cat. No. MABN1801, is a highly specific mouse monoclonal antibody that targets PMCA4b and has been tested in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected PMCA4b in Western Blotting applications (Caride, A.J., et. al. (1996). Biochem J. 316 (Pt 1):353-9; Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31; DeMarco, S.J., et. al. (2002). J Biol Chem. 277(12):10506-11; Gros, R., et. al. (2003). Circ Res. 93(7):614-21; Paszty, K., et. al. (2015). Sci Signal. 8(364):ra19; Usachev. M., et. al. (2001). J Neurochem. 76(6):1756-65).

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected PMCA4b in human cerebral cortex, human kidney, and bovine cornea tissues.

Immunohistochemistry Analysis: A representative lot detected PMCA4b in Immunohistochemistry applications (Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31).

Immunocytochemistry Analysis: A representative lot detected PMCA4b in Immunocytochemistry applications (Usachev. M., et. al. (2002). J Neuron. 33(1):113-22).

ELISA Analysis: A representative lot detected PMCA4b in ELISA applications (Caride, A.J., et. al. (1996). Biochem J. 316 (Pt 1):353-9; Borke, J.L., et. al. (1987). J clin Invest. 80(5):1225-31).
Biological Information
ImmunogenPurified plasma membrane calcium-transporting ATPase from human red blood cells.
CloneJA3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone JA3 detects Plasma membrane calcium-transporting ATPase in human brain tissue. It targets an epitope with in 25 amino acids from the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Chimpanzee
Species Reactivity NoteHuman, Chimpanzee.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ATP2B4
  • ATP2B2
  • MXRA1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~134 kDa observed; 137.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected PMCA4b in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1801-25UG 04054839512483