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AM80 Anti-PAR (Ab-1) Mouse mAb (10H)

This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).

Anti-PAR (Ab-1) Mouse mAb (10H) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Poly (ADP-Ribose)

AM80
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Dr, H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
AM80-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
Catalogue NumberAM80
Brand Family Calbiochem®
SynonymsAnti-Poly (ADP-Ribose)
References
ReferencesKawamitsu, H., et al. 1984. Biochemistry 23, 3771.
Product Information
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
Negative controlNormal mouse IgG
Positive controlEtoposide-treated HL-60 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (2.5 µg/ml)
Immunocytochemistry (5-20 µg/ml)
Application CommentsPAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
Biological Information
Immunogenpurified poly(ADP-ribose)
ImmunogenBovine
Clone10H
HostMouse
Purity≥95% by SDS-PAGE
IsotypeIgG₃
Species Reactivity
  • Drosophila
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AM80-100UG 04055977228052

Documentation

Anti-PAR (Ab-1) Mouse mAb (10H) SDS

Title

Safety Data Sheet (SDS) 

Anti-PAR (Ab-1) Mouse mAb (10H) Certificates of Analysis

TitleLot Number
AM80

References

Reference overview
Kawamitsu, H., et al. 1984. Biochemistry 23, 3771.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-August-2007 RFH
SynonymsAnti-Poly (ADP-Ribose)
ApplicationImmunoblotting (2.5 µg/ml)
Immunocytochemistry (5-20 µg/ml)
DescriptionProtein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
BackgroundThis monoclonal antibody is directed against poly(ADP)-ribose (PAR) which is synthesized by the nuclear DNA repair enzyme poly(ADP)-ribose polymerase (PARP). PARP is selectively activated when DNA strands break and catalyze the addition of long branched chains of PAR to a variety of nuclear proteins, such as PARP. The amount of PAR formed in living cells with DNA damage is directly related to the extent of the damage. When DNA is damaged, PAR undergoes a rapid turnover, with a half-life only minutes long, as it is rapidly hydrolyzed and converted to free ADP-ribose by the enzyme poly(ADP)-ribose glycohydrolase (PARG). Upon DNA damage by γ-irradiation or oxidative stress PAR fluorescence can be detected for approximately 10 min. PARP is strongly activated during the so-called execution phase of apoptosis, causing a second round of PAR synthesis. PAR synthesized early in apoptosis is remarkably stable and its immunofluorescence correlates well with other markers of apoptosis.
HostMouse
Immunogen speciesBovine
Immunogenpurified poly(ADP-ribose)
Clone10H
IsotypeIgG₃
SpeciesDrosophila, human, mouse, rat
Positive controlEtoposide-treated HL-60 cells
Negative controlNormal mouse IgG
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥95% by SDS-PAGE
Preservative≤0.1% sodium azide
CommentsPAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKawamitsu, H., et al. 1984. Biochemistry 23, 3771.