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MABF28 Anti-PAR-3 Antibody, clone 8E8

Anti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA.

Anti-PAR-3 Antibody, clone 8E8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Infectious Diseases Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: F2RL2, PAR3 UniProt Number: O00254
MABF28
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HFC, Enzyme AssaysMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF28
ReplacesMABS174
DescriptionAnti-PAR-3 Antibody, clone 8E8
Alternate Names
  • Proteinase-activated receptor 3
  • Coagulation factor II receptor-like 2
  • Thrombin receptor-like 2
Background InformationPAR-3 (also known as Coagulation factor II receptor-like 2, Thrombin receptor-like 2 or F2RL2) is an adapter protein involved in asymmetrical cell division and cell polarization processes. It seems to play a central role in the formation of epithelial tight junctions. Its association with PARD6B may prevent the interaction of PAR-3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PAR-3 complex links GTP bound Rho small GTPases to atypical protein kinase C proteins. PAR-3 interacts with PARD6A and PARD6B. Isoform 2, but not at least isoform 3 interacts with PRKCZ. PAR-3 interacts with PRCKI. PAR-3 forms part of a complex with PARD6A or PARD6B, PRKCI or PRKCZ and CDC42 or RAC1. PAR-3 interacts with F11R/JAM1. Antibodies against PAR-3 are present in sera from patients with cutaneous T cell lymphomas.
References
Product Information
FormatPurified
HS Code3002 15 90
Control
  • Mouse platelets from washed whole blood (heparinized)
PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PAR-3 Antibody, clone 8E8 detects level of PAR-3 & has been published & validated for use in FC, EA.
Key Applications
  • Flow Cytometry
  • Enzyme Assays
Application NotesFlow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)

Activity Assay Analysis (platelet aggregation): A previous lot was used by an independent laboratory in platelet aggregation assay. (Petrova, Y., et al. (2008). Centr Eur J Immunol. 33 (1):14-18.)
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human PAR-3.
Clone8E8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteDemonstrated to react with mouse.
Predicted to react with human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCoagulation factor II (thrombin) receptor-like 2 (F2RL2) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL2 is also a member of the protease-activated receptor family and activated by thrombin. F2RL2 is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. F2RL2 is a cofactor for F2RL3 activation by thrombin. It mediates thrombin-triggered phosphoinositide hydrolysis and is expressed in a variety of tissues. [provided by RefSeq].
Gene Symbol
  • F2RL2
  • PAR3
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.

SUBUNIT STRUCTURE: Interacts with INSC/inscuteable and probably GPSM2.

SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

TISSUE SPECIFICTY: Highest expression in the megakaryocytes of the bone marrow, lower in mature megakaryocytes, in platelets and in a variety of other tissues such as heart and gut. PTM: A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family.

SEQUENCE CAUTION: The sequence CAD97628.1 differs from that shown. Reason: Frameshift at position 374.
Molecular Weight40 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse platelets from washed whole blood (heparinized).

Flow Cytometry Analysis: 2 µg of this antibody detected PAR-3 in mouse platelets from washed whole blood (heparinized).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF28 04053252413568

Documentation

Anti-PAR-3 Antibody, clone 8E8 SDS

Title

Safety Data Sheet (SDS) 

Anti-PAR-3 Antibody, clone 8E8 Certificates of Analysis

TitleLot Number
Anti-PAR-3, clone 8E8 - 2449224 2449224
Anti-PAR-3, clone 8E8 - 2051419 2051419
Anti-PAR-3, clone 8E8 - 2512105 2512105
Anti-PAR-3, clone 8E8 - 3124158 3124158
Anti-PAR-3, clone 8E8 - 3230972 3230972
Anti-PAR-3, clone 8E8 - 3387606 3387606
Anti-PAR-3, clone 8E8 - 3955344 3955344
Anti-PAR-3, clone 8E8 - 4036266 4036266
Anti-PAR-3, clone 8E8 - 4133106 4133106
Anti-PAR-3, clone 8E8 - NG1895545 NG1895545
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