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MABS1254 Anti-O-GlcNAc Antibody, clone CTD110.6

Anti-O-GlcNAc Antibody, clone CTD110.6 is an antibody against O-GlcNAc for use in Western Blotting, ELISA, Immunoprecipitation.

Anti-O-GlcNAc Antibody, clone CTD110.6 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification Gene Symbol: N/A UniProt Number: N/A
MABS1254
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AWB, ELISA, IPMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1254
DescriptionAnti-O-GlcNAc Antibody, clone CTD110.6
Alternate Names
  • O-GlcNAc
  • beta-O-GlcNAc
  • O-Linked N-Acetylglucosamine
  • beta-O-linked N-acetylglucosamine
Background InformationPosttranslational modification of proteins by β-linked N-acetylglucosamine (β-GlcNAc) via the hydroxyl moieties on serine or threonine residues is termed O-linked β-GlcNAc or simply O-GlcNAc. O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all animals and plants. Unlike other types of protein glycosylations, O-GlcNAc occurs exclusively within the nuclear and cytoplasmic compartments and is generally not further modified to form more elongated structures. In addition, O-GlcNAcylation is a highly dynamic and reversible process. The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues, while O-GlcNAcase catalyzes the removal/hydrolysis of O-GlcNAc from proteins. In fact, a dynamic interplay between O-GlcNAcylation and serine/threonine phosphorylation plays an important role in regulating cellular signaling. Tau and RNA polymerase II (Pol II) are two well known proteins that undergo modification by O-GlcNAcylation. In Alzheimer’s diseased human brains, tau becomes extensively phosphorylated and less O-GlcNAcylated. Similarly, O-GlcNAc is removed and replaced with O-phosphate on the Poly II CTD when the elongation phase of transcription is initiated.
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgMκ antibody in PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-O-GlcNAc Antibody, clone CTD110.6 is an antibody against O-GlcNAc for use in Western Blotting, ELISA, Immunoprecipitation.
Key Applications
  • Western Blotting
  • ELISA
  • Immunoprecipitation
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected O-GlcNAcylated proteins in 7.5-15 µg of wild-type mouse embryonic fibroblast (MEF) lysate, but not O-GlcNAc transferase/OGT-deficient MEF lysate (Courtesy of Dr. Natasha Zachara and Gokben Yildirir, M.S.).
ELISA Analysis: A representative lot detected RNA polymerase II C-terminal domain (CTD) peptide (YSPTSPS) with a single O-GlcNAcylated serine or threonine, but not the corresponding unmodified peptide (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
Immunoprecipitation Analysis: A representative lot immunoprecipitated O-GlcNAcylated proteins from human pluripotent stem cells (hPSCs) (Maury, J.J., et al. (2013). Stem Cell Res. 11(2):926-937).
Immunoprecipitation Analysis: A representative lot immunoprecipitated O-GlcNAcylated proteins from HeLa cell extracts (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
Western Blotting Analysis: A representative lot detected similar level of cellular O-GlcNAcylation in undifferentiated, differentiating and terminally differentiated human pluripotent stem cells (hPSCs) (Maury, J.J., et al. (2013). Stem Cell Res. 11(2):926-937).
Western Blotting Analysis: A representative lot detected BSA-conjugated RNA polymerase II C-terminal domain (CTD) peptide (YSPTSPS) with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc, nor the corresponding unmodified peptide. The presence of GlcNAc, but not GalNAc, abolished the detection of the target bands (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
Western Blotting Analysis: A representative lot detected O-GlcNAcylated proteins in HeLa nuclear extract, as well as O-GlcNAcylated proteins purified from HeLa nuclear & cytosolic extract by wheat germ agglutinin (WGA) column. Antibody blocking by mmunogen peptide prior to immunoblotting abolished target bands detection (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
Western Blotting Analysis: A representative lot detected an upregulation of O-GlcNAcylated proteins in Jurkat cells treated with the glucosaminidase inhibitor PUGNAc and the hexosamine pathway intermediate glucosamine (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
Biological Information
ImmunogenKLH-conjugated peptide with an O-GlcNAc-modified serine residue.
Epitopebeta-O-linked GlcNAc.
CloneCTD110.6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). Anal. Biochem. 293(2):169-177).
IsotypeIgMκ
Species Reactivity
  • All
Species Reactivity NoteAll Species. Target modification is not species-specific.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • N/A
UniProt Number
Molecular WeightVariable, depending on the size(s) of the O-GlcNAcylated protein(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 4.0 µg/mL of this antibody detected O-GlcNAcylated proteins in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1254 04055977349610

Documentation

Anti-O-GlcNAc Antibody, clone CTD110.6 SDS

Title

Safety Data Sheet (SDS) 

Anti-O-GlcNAc Antibody, clone CTD110.6 Certificates of Analysis

TitleLot Number
Anti-O-GlcNAc, clone CTD110.6 - 3237506 3237506
Anti-O-GlcNAc, clone CTD110.6 - 3280062 3280062
Anti-O-GlcNAc, clone CTD110.6 - 3485877 3485877
Anti-O-GlcNAc, clone CTD110.6 - 3590153 3590153
Anti-O-GlcNAc, clone CTD110.6 - 3722068 3722068
Anti-O-GlcNAc, clone CTD110.6 - 3811351 3811351
Anti-O-GlcNAc, clone CTD110.6 - 3907041 3907041
Anti-O-GlcNAc, clone CTD110.6 - 3932181 3932181
Anti-O-GlcNAc, clone CTD110.6 - 4146094 4146094
Anti-O-GlcNAc, clone CTD110.6 - 4209502 4209502
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