Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

06-172-I Anti-Na+/K+ ATPase alpha-3

Anti-Na+/K+ ATPase alpha-3, Cat. No. 06-172-I, is a highly specific rabbit polyclonal antibody that targets Sodium/potassium-transporting ATPase subunit alpha-3 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Na+/K+ ATPase alpha-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Atp1a3 UniProt Number: P06687
06-172-I
200 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)RbPurifiedPolyclonal Antibody
Description
Catalogue Number06-172-I
DescriptionAnti-Na+/K+ ATPase alpha-3
Alternate Names
  • ATP1A3
  • Na+/K+ ATPase alpha-3
  • Sodium/potassium-transporting ATPase subunit alpha-3
  • Na(+)/K(+) ATPase alpha-3 subunit
  • Na(+)/K(+) ATPase alpha(III) subunit
  • Sodium pump subunit alpha-3
Background InformationSodium/potassium-transporting ATPase subunit alpha-3 (UniProt: P06687; also known as EC: 3.6.3.9, Na(+)/K(+) ATPase alpha-3 subunit, Na(+)/K(+) ATPase alpha(III) subunit, Sodium pump subunit alpha-3) is encoded by the Atp1a3 gene (Gene ID: 24213) in rat. Sodium/potassium-transporting ATPase subunit alpha-3 is a multi-pass membrane protein that is exclusive to the brain tissue and is highly expressed in cell bodies and projections of hippocampus. It is responsible for establishing and maintaining the electrochemical gradients of sodium and potassium ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. Sodium/potassium-transporting ATPase subunit alpha-3 is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic component catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. Reduced levels of neuronal sodium/ potassium ATPase activity in ATP1A3 heterozygous mice increases their susceptibility to depression-like endophenotypes by chronic variable stress.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Na+/K+ ATPase alpha-3, Cat. No. 06-172-I, is a highly specific rabbit polyclonal antibody that targets Sodium/potassium-transporting ATPase subunit alpha-3 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution of this antibody detected Na+/K+ ATPase alpha-3 in human cerebral cortex and human cardiac muscle tissue.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 25 amino acids from the N-terminal region of rat Sodium/potassium-transporting ATPase subunit alpha-3.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Sodium/potassium-transporting ATPase subunit alpha-3 in human, mouse, and rat brain. It targets an epitope with in 25 amino acids from the N-terminal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Atp1a3
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~112 kDa observed; 111.75 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in adult human brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Na+/K+ ATPase alpha-3 in 10 µg of adult human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
06-172-I 04054839316067