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ABE1409 Anti-Lysine-Specific Demethylase 4C (JMJD2C)

Anti-Lysine-Specific Demethylase 4C (JMJD2C) Antibody, Cat. No. ABE1409, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 4C and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-Lysine-Specific Demethylase 4C (JMJD2C) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: KDM4C, GASC1, JHDM3C, JMJD2C, KIAA0780 UniProt Number: Q9H3R0
ABE1409
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MIH(P), WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1409
DescriptionAnti-Lysine-Specific Demethylase 4C (JMJD2C)
Alternate Names
  • Gene amplified in squamous cell carcinoma 1 protein
  • GASC-1 protein
  • JmjC domain-containing histone demethylation protein 3C
  • Jumonji domain-containing protein 2C
  • Jmjd2C
Background InformationLysine-specific demethylase 4C (UniProt: Q9H3R0; also known as EC: 1.14.11.-; Gene amplified in squamous cell carcinoma 1 protein, GASC-1 protein, JmjC domain-containing histone demethylation protein 3C, Jumonji domain-containing protein 2C, Jmjd2c) is encoded by the KDM4C (also known as GASC1, JHDM3C, JMJD2C, KIAA0780) gene (Gene ID: 23081) in human. Jmjd2c is an ubiquitously expressed histone demethylase that specifically demethylates Lys-9 and Lys-36 residues of histone H3. It is shown to demethylate only the trimethylated H3 and does not affect mono- and dimethylated residues. It does neither demethylate histone Lys-4 or Lys-27 of histone H3 nor the Lys-20 of histone H4. Human Jmjd2C contains one jumonji N domain (aa 16-58), a catalytic jumonji C domain (aa 144-310), two PHD zinc finger regions (aa 689-865), and two TUDOR methylated-histone binding domains (aa 877-991). Jmjd2c, along with its closely demethylase Jmjd2b, plays a vital role in self-renewal of embryonic stem cells (ESC) and in generation of induced pluripotent stem cells. Full-length Jmjd2b and Jmjd2c are shown to restore the normal ESC growth phenotype, ESC colony morphology, and also SSEA-1 expression. Jmjd2c is reported to assist polycomb repressive complex (PRC) in transcriptional repression. It is overexpressed in several esophageal squamous cell carcinomas.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Lysine-Specific Demethylase 4C (JMJD2C) Antibody, Cat. No. ABE1409, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 4C and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of C2C12 and HEK293 nuclear extract.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Lysine-Specific Demethylase 4C (JMJD2C) in human tonsil, human cervical cancer, and human liver cancer tissues.
Biological Information
ImmunogenKLH-Conjugated linear peptide corresponding to 18 amino acids from the N-terminal half of human Lysine-specific demethylase 4C.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects Lysine-specific demethylase 4C in multiple human and mouse cell types.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • KDM4C
  • GASC1
  • JHDM3C
  • JMJD2C
  • KIAA0780
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~120 kDa observed; 119.98 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in BALB/3T3 clone A31 cell nuclear extract.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-Specific Demethylase 4C (JMJD2C) in 10 µg of BALB/3T3 clone A31 nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1409 04054839201363