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MABF472 Anti-Ly-6G (Gr-1) Antibody (mouse), PE, clone RB6-8C5

This rat monoclonal antibody Anti-Ly-6G (Gr-1) (mouse), PE, clone RB6-8C5 validated for use in IHC & Flow Cytometry for the detection of Ly-6G (Gr-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Ly6g UniProt Number: P35461
MABF472
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIHC, FCRPEMonoclonal Antibody
Description
Catalogue NumberMABF472
DescriptionAnti-Ly-6G (Gr-1) Antibody (mouse), PE, clone RB6-8C5
Alternate Names
  • Lymphocyte antigen 6G
  • Ly-6G
  • Ly-6G.1
Background InformationThe RB6-8C5 antibody binds to mouse Ly-6G, commonly known as Gr-1, a member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Gr-1 is differentially expressed during development and maturation of cells in the myeloid lineage and is expression at varying stages and levels on monocytes, macrophages, granulocytes, and peripheral neutrophils. In the mouse, the RB6-8C5 antibody is typically used in combination with the macrophage labeling antibody M1/70 (Anti-CD11b) for phenotypic analysis of monocytes, macrophages and granulocytes.Note: The RB6-8C5 antibody has been reported to cross-react with Ly-6C on cells expressing this antigen (Fleming et al. 1993. J. Immunol. 151:2399-2408 and Sasmono et al. 2007. J. Leukoc. Biol. 82: 111-123) and has been cited in the literature for identification of Ly-6G/Ly-6C. Other reports sµggest that this antibody is specific for Ly-6G, without cross-reactivity for Ly-6C (Nagendra S. and Schlueter AJ. 2003. Cytometry A, 58(2): 195-200.
Product Information
FormatPE
PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal antibody Anti-Ly-6G (Gr-1) (mouse), PE, clone RB6-8C5 validated for use in IHC & Flow Cytometry for the detection of Ly-6G (Gr-1).
Key Applications
  • Immunohistochemistry
  • Flow Cytometry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneRB6-8C5
Concentration0.2 mg/mL
HostRat
IsotypeIgG2bκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Ly6g
Purification MethodSize Exclusion Chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected Ly-6G (Gr-1) in one million C57Bl/6 bone marrow cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MABF472 04053252962424