Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABN2268-25UG Anti-LRRK1 Antibody, clone 12F2.1

Anti-LRRK1, clone 12F2.1, Cat. No. MABN2268, is a highly specific mouse monoclonal antibody that detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category: Neuroscience Gene Symbol: LRRK1, KIAA790 UniProt Number: Q38SD2
MABN2268-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2268-25UG
DescriptionAnti-LRRK1 Antibody, clone 12F2.1
Alternate Names
  • Leucine-rich repeat serine/threonine-protein kinase 1
  • Leucine-rich repeat kinase 1
Background InformationLeucine-rich repeat serine/threonine-protein kinase 1 (UniProt: Q38SD2, Leucine-rich repeat kinase 1) is encoded by the LRRK1 (also known as KIAA1790) gene (Gene ID: 79705) in human. Leucine-rich repeat kinase 1 is a member of the ROCO family of protein kinases. Two isoforms of Leucine-rich repeat kinase 1 have been described that are generated by alternative splicing. It contains a ROC (Ras in complex proteins) domain together with a MAPKKK-like kinase domain and several protein protein interactions. It is reported to form a complex with activated EGFR via interaction with Grb2. Subsequently, it is internalized to early endosomes along with EGFR. Leucine-rich repeat kinase 1 also regulates EGFR transport from early to late endosomes and is involved in regulating the motility of EGF-containing early endosomes that is dependent on its kinase activity. Leucine-rich repeat kinase 1 is also reported to play a role in regulating bone mass and mice with defective gene are prone to osteoporosis, increased bone mineralization, and diminished bone resorption. (Ref.: Hanafusa, H et al. (2011). Nature Commun. 2, Article number: 158).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-LRRK1, clone 12F2.1, Cat. No. MABN2268, is a highly specific mouse monoclonal antibody that detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected LRRK1 in human cerebral cortex, human testis, and human pancreatic cancer tissues.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 86 amino acids from the internal region of human Leucine-rich repeat serine/threonine-protein kinase 1.
Clone12F2.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 12F2.1 detects Leucine-rich repeat serine/threonine-protein kinase 1 (LRRK1) in human. It targets an epitope within 86 amino acids from the internal region.
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • LRRK1
  • KIAA790
Purification MethodProtein G purified
UniProt Number
Molecular Weight~200 kDa observed; 225.39 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human hypothalamus tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected LRRK1 in 10 µg of human hypothalamus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2268-25UG 04054839522093

Documentation