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MABE1879-25UG Anti-Ku70 Antibody, clone N3H10

Anti-Ku70, clone N3H10, Cat. No. MABE1879, is a mouse monoclonal antibody that detects X-ray repair cross-complementing protein 6 (Ku70) and is tested for use in Immunocytochemistry and Western Blotting.

MABE1879-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1879-25UG
DescriptionAnti-Ku70 Antibody, clone N3H10
Alternate Names
  • X-ray repair cross-complementing protein 6
  • 5'-deoxyribose-5-phosphate lyase Ku70
  • 5'-dRP lyase Ku70
  • 70 kDa subunit of Ku antigen
  • ATP-dependent DNA helicase 2 subunit 1
  • ATP-dependent DNA helicase II 70 kDa subunit
  • CTC box-binding factor 75 kDa subunit
  • CTC75
  • CTCBF
  • DNA repair protein XRCC6
  • Lupus Ku autoantigen protein p70
  • Thyroid-lupus autoantigen
  • TLAA
  • X-ray repair complementing defective repair in Chinese hamster cells 6
Background InformationX-ray repair cross-complementing protein 6 (UniProt: P12956; also known as 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, DNA repair protein XRCC6. Lupus Ku autoantigen protein p70, Ku70, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 6) is encoded by the XRCC6 (also known as G22P1) gene (Gene ID: 2547) in human. Ku autoantigen is a heterodimeric protein composed of Ku70 and Ku80. Ku70 is an evolutionarily conserved protein that binds to DNA double-strand break ends and helps repair DNA via the non-homologous end-joining (NHEJ) pathway.
The Ku70/Ku80 dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Ku70 serves as a cytosolic DNA sensor that induces the production of IFN- 1, a type III IFN, in primary human cell lines after viral infection after translocating from nucleus to cytosol to interact with STING. (Ref.: Fell, VL., and Schild-Poulter, C. (2012). Mol. Cell Biol. 32(1); 76-87; Sui, H., et al. (2017). Sci. Signaling 10(488); eaah5054).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-Ku70, clone N3H10, Cat. No. MABE1879, is a mouse monoclonal antibody that detects X-ray repair cross-complementing protein 6 (Ku70) and is tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected Ku70 in Western Blotting applications (Knuth, M.W., et. al. (1990). J Biol Chem. 265(29):17911-20).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified Protein corresponding to Human X-ray repair cross-complementing protein 6 (Ku70).
EpitopeC-terminal half
CloneN3H10
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone N3H10 is a mouse monoclonal antibody that detects Human Ku70 protein. It targets an epitope within the C-terminal half.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • XRCC6
  • G22P1
Purification MethodProtein G purified
UniProt Number
Molecular Weight69.84 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in PC3 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Ku70 in PC3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1879-25UG 04065265324212