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AB1413-I Anti-Interleukin-1β

This rabbit polyclonal Anti-Interleukin-1 Antibody, Cat. No. AB1413-I is validated for use in Western Blotting for the detection of Interleukin-1 .

Anti-Interleukin-1β MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Il1b UniProt Number: P10749
AB1413-I
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB1413-I
ReplacesAB1413
DescriptionAnti-Interleukin-1β
Alternate Names
  • Interleukin-1 beta
  • IL-1 beta
  • IL-1b
  • IL-1beta
Background InformationInterleukin-1 beta (UniProt; also known as IL-1 beta, IL-1b, IL-1beta) is encoded by the Il1b gene (Gene ID 16176) in murine species. Interleukin-1 (IL-1) is a cytokine that plays a central role in the regulation of acute and chronic inflammation. Two forms of IL-1, IL-1 and IL-1 , are transcribed from two separate genes and mediate similar, but not completely overlapping, biological effects via the membrane-bound IL-1 type 1 receptor (IL-1R1). Studies utilizing Il1a- and Il1b-knockout mice showed that IL-1 , but not IL-1 , is involved in T-cell-dependent antibody production via antigen-specific T-cell activation. IL-1 binding to the extracellular domain of IL1R1 promotes the recruitment of the IL-1 receptor accessory protein and subsequent signal transduction mediated by the cytoplasmic domains of IL-1R1 and IL1-R2. The IL-1 type 2 receptor (IL1R2) is unable to initiate signaling and only acts as a decoy receptor. Soluble receptors for IL-1 (sIL-1Rs) are produced by proteolytic cleavage of membrane-bound receptors or by alternative splicing and act as natural inhibitors by competing against membrane-bound IL1Rs for IL-1 binding. Murine IL-1 beta is produced with a propeptide sequence (a.a. 1-117), which is removed posttranslationally to yield the mature (a.a. 118-269) protein.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rabbit polyclonal Anti-Interleukin-1 Antibody, Cat. No. AB1413-I is validated for use in Western Blotting for the detection of Interleukin-1 .
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected pro- and mature IL-1beta in 10 µg of lysates from mouse testis tissue and LPS-stimulated RAW264.7 cells, respectively.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a region within the C-terminal half of the mature murine and rat IL-1beta sequence.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detected both the ~17 kDa mature and ~30 kDa pro-form of murine IL-1beta. The corresponding sequence in human IL-1beta differs from that of the immunogen by only one amino acid. Immunogen sequence is not found in IL-1alpha.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Gerbil, Hamster, and Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Il1b
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~17/30 kDa observed. 17.39/30.93 kDa (mouse mature/pro-form), 17.32/30.64 kDa (rat mature/pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IL-1beta in 10 µg of NIH/3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
AB1413-I 04054839055102