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MABT1357-25UL Anti-IZUMO1 Antibody, clone Mab120

Anti-IZUMO1, clone Mab120, Cat. No. MABT1357, is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 and has been tested for use in Immunofluorescence, Inhibition assay, and Western Blotting.

Anti-IZUMO1 Antibody, clone Mab120 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Izumo1 UniProt Number: Q9D9J7
MABT1357-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIF, Inhibition, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1357-25UL
DescriptionAnti-IZUMO1 Antibody, clone Mab120
Alternate Names
  • Izumo sperm-egg fusion protein 1
  • Oocyte binding/fusion factor
  • OBF
  • Sperm-specific protein Izumo
Background InformationIzumo sperm-egg fusion protein 1 (UniProt: Q9D9J7; also known as Oocyte binding/fusion factor, OBF, Sperm-specific protein izumo) is encoded by the Izumo1 gene (Gene ID: 73456) in murine species. OBF is a single-pass type I membrane protein that is detected on sperm surface after the acrosome reaction. It is an essential sperm cell-surface protein required for fertilization by acting as a ligand for IZUMO1R/JUNO receptor on egg. It can be present as a monomer, homodimer, or homooligomer. It is synthesized with a signal peptide (aa 1-21), which is cleaved off in the mature form, which has an extracellular domain (aa 22-319), a short transmembrane domain (aa 320-340), and a cytoplasmic domain (aa 341-397). The extracellular domain assumes a distinct boomerang shape when not bound to IZUMO1R/JUNO. Its interaction with IZUMO1R/JUNO triggers a conformation change, so that the IZUMO1 extracellular domain assumes an upright conformation. A region comprising of 13 amino acids (148-160) is important for its interaction with IZUMO1R. Mab120 is a fusion-inhibiting antibody that inhibits fertilization. It binds to a region designated as putative functional fragment (aa 5-113). (Ref.: Inoue, N et al. (2013). Development. 140(15); 3221-3229).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in PBS without azide.
Applications
ApplicationAnti-IZUMO1, clone Mab120, Cat. No. MABT1357, is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 and has been tested for use in Immunofluorescence, Inhibition assay, and Western Blotting.
Key Applications
  • Immunofluorescence
  • Inhibition
  • Western Blotting
Application NotesInhibition Analysis: A representative lot inhibited fertilization in an in vitro system. (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Western Blotting Analysis: A representative lot detected IZUMO1 in Western Blotting applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).

Immunofluorescence Analysis: A representative lot detected IZUMO1 in Immunofluorescence applications (Inoue, N., et. al. (2013). Development. 140(15):3221-9).
Biological Information
ImmunogenMouse spermatozoa.
CloneMab120
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone Mab120 is a rat monoclonal antibody that detects Izumo sperm-egg fusion protein 1 (IZUMO1) in murine species. It targets an epitope with in the N-terminal putative functional fragment.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Izumo1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~56 kDa observed; 44.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected IZUMO1 in mouse testis tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1357-25UL 04054839563430