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MAB10009 Anti-IDO Antibody, clone 1F8.2

MAB10009
100 µg  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB10009
DescriptionAnti-IDO Antibody, clone 1F8.2
Alternate Names
  • Indoleamine-pyrrole 2,3-dioxygenase
  • indole 2,3-dioxygenase
  • indoleamine 2,3-dioxygenase 1
  • indoleamine-pyrrole 2,3 dioxygenase
  • IDO1
Background InformationIndoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the
suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
Product Information
FormatPurified
Control
  • HeLa cell lysate treated with interferon gamma.
PresentationMouse monoclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 150 mM NaCl and 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-IDO Antibody, clone 1F8.2 is an antibody against IDO for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Biological Information
ImmunogenGST-tagged recombinant protein of full-length human IDO.
Clone1F8.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes IDO.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteProven to react with human.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD107B
  • EC 1.13.11.52
  • IDO
  • INDO
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.
CATALYTIC ACTIVITY: D-tryptophan + O2 = N-formyl-D-kynurenine.
L-tryptophan + O2 = N-formyl-L-kynurenine.
COFACTOR: Binds 1 heme group per subunit.
INDUCTION: By interferon gamma.
SEQUENCE SIMILARITIES: Belongs to the indoleamine 2,3-dioxygenase family.
Molecular WeightApprox. 45 kDa
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 µg/mL dilution of this antibody detected IDO on 15 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C for 1 year from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MAB10009 04053252667589