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MABN1616 Anti-GADD153/DDIT3/CHOP Antibody, clone 5E5.3

This Anti-GADD153 Antibody, clone 5E5.3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of CHOP.

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Research Category: Neuroscience Research Sub-Category: Apoptosis - Additional Gene Symbol: DDIT3, CEBPZ, CHOP, CHOP10, GADD153 UniProt Number: P35638
MABN1616
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1616
DescriptionAnti-GADD153/DDIT3/CHOP Antibody, clone 5E5.3
Alternate Names
  • DNA damage-inducible transcript 3 protein
  • CCAAT/enhancer-binding protein homologous protein
  • C/EBP-homologous protein 10
  • C/EBP zeta
  • CHOP
  • CHOP-10
  • DDIT-3
  • Growth arrest and DNA damage-inducible protein GADD153
Background InformationDNA damage-inducible transcript 3 protein (UniProt P35638; also known as CCAAT/enhancer-binding protein homologous protein, C/EBP-homologous protein 10, C/EBP zeta, CHOP, CHOP-10, DDIT-3, Growth arrest and DNA damage-inducible protein GADD153) is encoded by the DDIT3 (Also known as CEBPZ, CHOP, CHOP10, GADD153) gene in human (Gene ID 1649). CHOP is a proapoptotic unfold protein response (UPR) gene and a well established marker for ER stress and UPR activation. The accumulation of unfolded or misfolded proteins in the ER induces the activation of three main UPR pathways, the inositol requiring enzyme-1 (IRE1) pathway, the activating transcription factor 6 (ATF6) pathway, and the protein kinase-like ER kinase (PERK) pathway. The PERK pathway involves PERK auto-phosphorylation/activation, resulting in PERK phosphorylation of eIF2α, which in turn leads to increased ATF4 transcription and ATF4-mediated CHOP upregulation.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-GADD153 Antibody, clone 5E5.3 is validated for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin) for the detection of CHOP.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry (Paraffin)
Application NotesImmunocytochemistry Analysis: 5.0 µg/mL from a representative lot detected GADD153/DDIT3 in HeLa cells.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GADD153/DDIT3 in human brain, kidney, and skeletal muscle tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human GADD153/DDIT3/CHOP internal fragment.
EpitopeInternal.
Clone5E5.3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 5E5.3 targeted region is present in both human GADD153/DDIT3/CHOP spliced isoforms reported by UniProt (P35638).
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • DDIT3
  • CEBPZ
  • CHOP
  • CHOP10
  • GADD153
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~20 kDa observed. 19.18 kDa (isoform 1) and 21.70 kDa (isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting Analysis in HUVEC lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GADD153/DDIT3 in 10 µg of HUVEC lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1616 04055977351514