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AB5459 Sigma-AldrichAnti-GABA A Receptor α4 Antibody, CT

Anti-GABA A Receptor α4 Antibody, C-terminus is an antibody against GABA A Receptor α4 for use in IP & WB.

Anti-GABA A Receptor α4 Antibody, CT MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References

Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: GABRA4, GABA UniProt Number: P48169

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M, R	IP, WB	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB5459
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-GABA A Receptor α4 Antibody, CT

References

Product Information
Format	Affinity Purified
Presentation	Affinity purified immunoglobulin in 10 mM HEPES, pH 7.5, with 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Quality Level	MQ100

Applications
Application	Anti-GABA A Receptor α4 Antibody, C-terminus is an antibody against GABA A Receptor α4 for use in IP & WB.
Key Applications	Immunoprecipitation Western Blotting
Application Notes	Western blot: 1:1,000 Immunopreciptation: The antibody will immunoprecipitate 8% of all GABA A receptors in sodium dodecyl sulfate extracts of rat forebrains (150 μL) using 20 μg of antibody. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	MBP fusion protein from the cytosolic loop of rat GABA A receptor alpha4 subunit.
Epitope	C-terminus
Concentration	Please refer to the Certificate of Analysis for the lot-specific concentration.
Host	Rabbit
Specificity	GABA A receptor alpha4 subunit. The apparent molecular mass of the alpha 4 subunit in rat brain membrane fractions is 64 kDa.
Species Reactivity	Human Mouse Rat
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_000809.2
Entrez Gene Summary	GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
Gene Symbol	GABRA4 GABA
UniProt Number	P48169
UniProt Summary	FUNCTION: SwissProt: P48169 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel. SIZE: 554 amino acids; 61640 Da SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P48169 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.

Packaging Information
Material Size	100 µL

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalogue Number	GTIN
AB5459	04053252408083

Documentation

Anti-GABA A Receptor α4 Antibody, CT SDS

Title
Safety Data Sheet (SDS)

Anti-GABA A Receptor α4 Antibody, CT Certificates of Analysis

Title	Lot Number
RABBIT ANTI-GABAA RECEPTOR ALPHA4 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2449220	2449220
RABBIT ANTI-GABAA RECEPTOR ALPHA4 - 3190908	3190908
RABBIT ANTI-GABAA RECEPTOR ALPHA4 - 3856684	3856684
RABBIT ANTI-GABAA RECEPTOR ALPHA4 - 4016253	4016253
RABBIT ANTI-GABAA RECEPTOR ALPHA4 - 4030500	4030500
RABBIT ANTI-GABAA RECEPTOR ALPHA4 - 4146073	4146073
RABBIT ANTI-GABAA RECEPTOR ALPHA4 -2798364	2798364
RABBIT ANTI-GABAA RECEPTOR ALPHA4 AFFINITY PURIFIED POLYCLONAL ANTIBODY	2925263
RABBIT ANTI-GABAA RECEPTOR ALPHA4 AFFINITY PURIFIED POLYCLONAL ANTIBODY	3088415
RABBIT ANTI-GABAA RECEPTOR ALPHA4 AFFINITY PURIFIED POLYCLONAL ANTIBODY	2986579

References

Reference overview	Pub Med ID
mRNA and protein levels for GABAAalpha4, alpha5, beta1 and GABABR1 receptors are altered in brains from subjects with autism. Fatemi, SH; Reutiman, TJ; Folsom, TD; Rooney, RJ; Patel, DH; Thuras, PD Journal of autism and developmental disorders 40 743-50 2010 Show Abstract We have shown altered expression of gamma-aminobutyric acid A (GABA(A)) and gamma-aminobutyric acid B (GABA(B)) receptors in the brains of subjects with autism. In the current study, we sought to verify our western blotting data for GABBR1 via qRT-PCR and to expand our previous work to measure mRNA and protein levels of 3 GABA(A) subunits previously associated with autism (GABRalpha4; GABRalpha5; GABRbeta1). Three GABA receptor subunits demonstrated mRNA and protein level concordance in superior frontal cortex (GABRalpha4, GABRalpha5, GABRbeta1) and one demonstrated concordance in cerebellum (GABBetaR1). These results provide further evidence of impairment of GABAergic signaling in autism.	20066485

Reference overview

Pub Med ID

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