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MAB038-I Anti-Factor VIII Antibody, 83 kDa light chain Antibody, clone F8C 27.4

Anti-Factor VIII, 83 kDa light chain, clone F8C 27.4, Cat. No. MAB038-I, is a mouse monoclonal antibody that detects Factor VIII, 83 kDa light chain and is tested for use in ELISA and Western Blotting

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MAB038-I
100 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMAB038-I
DescriptionAnti-Factor VIII Antibody, 83 kDa light chain Antibody, clone F8C 27.4
Alternate Names
  • Coagulant Component
  • Antihemophilic Factor
  • AHF
Background InformationCoagulation factor VIII (UniProt: P00451; also known as Antihemophilic factor, AHF, Procoagulant component) is encoded by the F8 (also known as F8C) gene (Gene ID: 2157) in human. Factor VIII is a heavily glycosylated plasma protein that is synthesized with a signal peptide (aa 1-19) and is further cleaved and processed to generate Factor VIIIa heavy chain of 200 kDa (aa 20-1332), Factor VIIIa heavy chain of 92 kDa (aa 20-759), Factor VIII B chain (aa 760-1332), and the Factor VIIIa light chain (aa 1668-2351). In plasma, Factor VIII is thought to be associated in a complex with the highest molecular weight multimers of Von Willebrand protein in an inactive form and following injury or wound it dissociates from Willebrand factor and is activated (Factor VIIIa). Along with calcium and phospholipid, it acts as a cofactor for Factor IXa, which is responsible for converting Factor X to Xa. Its concentration in plasma ranges from 100-200 ng/ml.
Product Information
FormatPurified
PresentationPurified mouse monoclonal in buffer containing PBS, pH 7.4, containing no preservatives.
Quality LevelMQ100
Applications
ApplicationAnti-Factor VIII, 83 kDa light chain, clone F8C 27.4, Cat. No. MAB038-I, is a mouse monoclonal antibody that detects Factor VIII, 83 kDa light chain and is tested for use in ELISA and Western Blotting.
Key Applications
  • ELISA
  • Western Blotting
Application NotesELISA (indirect): (Dilution is assay based)

Western blotting (Dilution is assay based)


Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenPurified Human Factor VIII.
EpitopeC-terminal half
CloneF8C 27.4
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone F8C 27.4 is a mouse monoclonal antibody that detects human Coagulation Factor VIII, 83 kDa light chain.
IsotypeIgG2a
Species Reactivity
  • Human
Species Reactivity NoteHuman
Antibody TypeMonoclonal Antibody
Gene Symbol
  • F8
  • F8C
Purification MethodProtein G purified
UniProt Number
Molecular Weight267 kDa calculated for full-length Coagulation Factor VIII.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
MAB038-I 04065265651547