Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABS2026-25UG Anti-EphA2 Antibody, clone F2-27

Anti-EphA2, clone F2-27, Cat. No. MABS2026, is a mouse monoclonal antibody that detects Ephrin type-A receptor 2 and has been tested for use in Flow Cytometry, Immunoprecipitation, and Inhibition Assay.

Anti-EphA2 Antibody, clone F2-27 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: EPHA2, ECK UniProt Number: P29317
MABS2026-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IP, InhibitionMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS2026-25UG
DescriptionAnti-EphA2 Antibody, clone F2-27
Alternate Names
  • Ephrin type-A receptor 2
  • EC: 2.7.10.1
  • Epithelial cell kinase
  • Tyrosine-protein kinase receptor ECK
Background InformationEphrin type-A receptor 2 (UniProt: P29317; also known as EC: 2.7.10.1, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK) is encoded by the EPHA2 (also known as ECK) gene (Gene ID: 1969) in human. Ephrin type-A receptor 2 is a homodimeric single-pass type I membrane protein that is expressed in brain and glioma tissue and in glioma cell lines. It is also highly expressed in tissues that contain a high proportion of epithelial cells. Its levels are shown to be up-regulated by UV irradiation via a TP53-independent, MAPK-dependent mechanism. Ephrin type-A receptor 2 serves as a receptor tyrosine kinase that binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. It is involved in the regulation of migration, integrin-mediated adhesion, and in proliferation and differentiation of cells. Ephrin type-A receptor 2 is phosphorylated on tyrosine residues upon binding and activation by EFNA1. Phosphorylation on Tyr 588 and Tyr 594 is reported to be essential for binding VAV2 and VAV3 while phosphorylation on Tyr 735 and Tyr 930 are required for binding PI3-kinase p85 subunit. It is synthesized with a signal peptide (aa 1-23) that is cleaved off in the mature form. The mature form has an extracellular domain (aa 24-537), a short transmembrane domain (aa 538-558), and a cytoplasmic domain (aa 559-976). Two isoforms of Ephrin type-A receptor 2 have been described that are produced by alternative splicing. EphA2 is reported to be overexpressed in skin tumors murine models and its overexpression may represent a compensatory feedback mechanism during tumorigenesis. (Ref.: Guo, H., et al. (2006). Cancer Res. 66(14); 7050-7058).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-EphA2, clone F2-27, Cat. No. MABS2026, is a mouse monoclonal antibody that detects Ephrin type-A receptor 2 and has been tested for use in Flow Cytometry, Immunoprecipitation, and Inhibition Assay.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Inhibition
Application NotesInhibition Analysis: A representative lot inhibited cancer cell invasion in a three-dimensional spheroid formation assay. (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).

Immunoprecipitation Analysis: A representative lot detected EphA2 in Immunoprecipitation applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).

Flow Cytometry Analysis: A representative lot detected EphA2 in Flow Cytometry applications (Tanaka, T., et. al. (2017). Transl Oncol. 10(4):476-484).
Biological Information
ImmunogenKP-2, KP-3, SUIT-2, and MIAPaCa-2 cells.
CloneF2-27
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone F2-27 specifically detects Ephrin type -A receptor 2 in human.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EPHA2
  • ECK
Purification MethodProtein G purified
UniProt Number
Molecular Weight108.27 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in PC-3 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected EphA2 in one million PC-3 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2026-25UG 04054839577222