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ABC1389-25UL Anti-DCLK1-S

Anti-DCLK1-S, Cat. No. ABC1389, is a rabbit polyclonal antibody that specifically detects the short version of Serine/threonine-protein kinase DCLK1. It has been tested for use in Immunohistochemistry and Western Blotting.

Anti-DCLK1-S MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: DCLK1, DCAMKL1, ;DCDC3A, KIAA0369, DCLK1 UniProt Number: O15075
ABC1389-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HIHC, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABC1389-25UL
DescriptionAnti-DCLK1-S
Alternate Names
  • Serine/threonine-protein kinase DCLK1-S
  • EC: 2.7.11.1
  • Doublecortin domain-containing protein 3A-short
  • Doublecortin-like and CAM kinase-like 1
  • Doublecortin-like kinase 1
  • PS41014
Background InformationSerine/threonine-protein kinase DCLK1 (UniProt: O15075; also known as EC: 2.7.11.1, Doublecortin domain-containing protein 3A, Doublecortin-like and CAM kinase-like 1, Doublecortin-like kinase 1) is encoded by the DCLK1 (also known as DCAMKL1, DCDC3A, KIAA0369) gene (Gene ID: 9201) in human. DCLK1 is an integral component of plasma membrane and is highly expressed in fetal brain tissue and is also detectable in lung and liver. Adults show ubiquitous expression. It has two doublecortin domains (aa 57-143 and 186-269). Four isoforms of DCLK1 have been reported (in UniProt) that are produced by alternative splicing. DCLK1 expression is reported to be critical for growth of human colon cancer cells and its expression is up-regulated in response to growth factors resulting in hyperproliferation. A short isoform, DCLK1-S, has been reported in human colorectal cancer cells and in cancer stem cells, while normal colon cells express only the long isoform of DCLK1. Hence, DCLK1-S may serve as a specific marker for cancer stem cells and the long isoform indicates normal human cells. The short isoform displays about 98% sequence homology with the C-terminal domains of the long isoform, but differs from it by six amino acids at the N-terminal end. Higher expression of Dclk-1s has been linked to poor prognosis and survival rate in colorectal cancer subjects. (Ref.: Sarkar, S et al. (2017). Lab. Invest. doi:10. 1038/labinvest.2017.40).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.05% sodium azide.
Applications
ApplicationAnti-DCLK1-S, Cat. No. ABC1389, is a rabbit polyclonal antibody that specifically detects the short version of Serine/threonine-protein kinase DCLK1. It has been tested for use in Immunohistochemistry and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected DCLK1-S in Immunohistochemistry applications (Sarkar, S., et. al. (2017). Lab Invest. [Epub ahead of print]).

Western Blotting Analysis: A representative lot detected DCLK1-S in Western Blotting applications (Sarkar, S., et. al. (2017). Lab Invest. [Epub ahead of print]).
Biological Information
ImmunogenA multimeric scaffold of the 6 amino acids from the N-terminal region of the short version of human DCLK1 (DCLK1-S).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects the short version of Serine/threonine-protein kinase DCLK1 (DCLK1-S). It does not react with long isoforms. It targets an epitope within six amino acids from the N-terminus region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Gene Symbol
  • DCLK1
  • DCAMKL1
  • ;DCDC3A
  • KIAA0369
  • DCLK1
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~55 kDa observed. 47.60 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HCT116 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected DCLK1-S in 10 µg of HCT116 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1389-25UL 04054839518614