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ABE86 Anti-CHD-3 Antibody

Anti-CHD-3 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-3 also known as Chromodomain-helicase-DNA-binding protein 3, hZFH & has been validated in WB.

Anti-CHD-3 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: CHD3 UniProt Number: Q12873
ABE86
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE86
ReplacesMABE482
DescriptionAnti-CHD-3 Antibody
Alternate Names
  • Chromodomain-helicase-DNA-binding protein 3
  • CHD-3
  • ATP-dependent helicase CHD3
  • Mi-2 autoantigen 240 kDa protein
  • Mi2-alpha
  • Zinc finger helicase
  • hZFH
Background InformationChromodomain-helicase-DNA-binding protein 3, more commonly known as CHD-3, is a member of the CHD family of proteins. These proteins are characterized by the presence of chromo (chromatin organization modifier) domain, SNF2-related helicase/ATPase domain, and and a carboxy-terminal DNA binding domain. As a component of the Mi-2/NuRD histone deacetylase complex, CHD-3 participates in chromatin remodeling by deacetylating histones.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-CHD-3 Antibody is a Rabbit Polyclonal Antibody for detection of CHD-3 also known as Chromodomain-helicase-DNA-binding protein 3, hZFH & has been validated in WB.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human CHD-3.
EpitopeC-terminus
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes CHD-3 at the C-terminus.
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq].
Gene Symbol
  • CHD3
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.

SUBUNIT STRUCTURE: Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity.

SUBCELLULAR LOCATION: Nucleus. Cytoplasm › cytoskeleton › centrosome. Note: Associates with centrosomes in interphase and mitosis.

TISSUE SPECIFICTY: Widely expressed.

MISCELLANEOUS: One of the main antigens reacting with anti-MI-2 positive sera of dermatomyositis.

SEQUENCE SIMILARITIES: Belongs to the SNF2/RAD54 helicase family.

Contains 2 chromo domains.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 2 PHD-type zinc fingers.

SEQUENCE CAUTION: The sequence AAB87383.1 differs from that shown. Reason: Differs from position 1967 onward for unknown reasons.
Molecular Weight~ 250 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.05 µg/mL of this antibody detected CHD-3 on 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE86 04053252685095