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MABF1983-25UG Anti-CD8a Antibody, clone YTS 169.4

Anti-CD8a, clone YTS 169.4, Cat. No. MABF1983 is a rat monoclonal antibody that detects T-cell surface glycoprotein CD8 alpha chain and has been tested for use in Flow Cytometry, Immunohistochemistry, and Immunodepletion.

Anti-CD8a Antibody, clone YTS 169.4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cd8a UniProt Number: P01731
MABF1983-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IHC, ImmunodepletionRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1983-25UG
DescriptionAnti-CD8a Antibody, clone YTS 169.4
Alternate Names
  • T-cell surface glycoprotein CD8 alpha chain
  • T-cell surface glycoprotein Lyt-2
  • CD_antigen CD8a
Background InformationT-cell surface glycoprotein CD8 alpha chain (UniProt: P01731; also known as T-cell surface glycoprotein Lyt-2, CD8a) is encoded by the Cd8a (also known as Lyt-2, Lyt2) gene (Gene ID: 12525) in murine species. CD8 is a single-pass type I membrane protein with one Ig-like V-type (immunoglobulin-like) domain. CD8a is produced with a 27 amino acid signal peptide that is subsequently cleaved. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor for antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2b in PBS without preservatives.
Applications
ApplicationAnti-CD8a, clone YTS 169.4, Cat. No. MABF1983 is a rat monoclonal antibody that detects T-cell surface glycoprotein CD8 alpha chain and has been tested for use in Flow Cytometry, Immunohistochemistry, and Immunodepletion.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry
  • Immunodepletion
Application NotesImmunodepletion Analysis: A representative lot detected CD8a in Immunodepletion applications (Cobbold, S.P., et. al. (1984). Nature. 312(5994):548-51; Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202; Brown, C.R., et. al. (1990). J Immunol. 145(10):3438-41).

Flow Cytometry Analysis: A representative lot detected CD8a in Flow Cytometry applications (Noort, W.A., et. al. (1996). Transpl Immunol. 4(3):198-202).

Immunohistochemistry Analysis: A representative lot detected CD8a in Immunohistochemistry applications (Wright, J.R. Jr, et. al. (1997). Ann Transplant. 2(3):12-6).
Biological Information
ImmunogenThymocytes from CBA mouse.
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone YTS 169.4 detects T-cell surface glycoprotein CD8 alpha chain (CD8a) in mouse thymocytes.
IsotypeIgG2b
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Cd8a
Purification MethodProtein G purified
UniProt Number
Molecular Weight27.5 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mouse thymocytes.

Flow Cytometry Analysis: 1 µg of this antibody detected CD8a in mouse thymocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF1983-25UG 04054839476921