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CBL517 Anti-CD28 Antibody, clone 15E8

This Anti-CD28 Antibody, clone 15E8 is validated for use in FC, FUNC for the detection of CD28.

Anti-CD28 Antibody, clone 15E8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: CD28, Tp44, TP44, MGC138290 UniProt Number: P10747
CBL517
100 µg  
Purchase on Sigma-Aldrich

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, FUNCMPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL517
ReplacesCBL517P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-CD28 Antibody, clone 15E8
Alternate Names
  • Tp44
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPlease see lot specific datasheet for product formulation. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Quality LevelMQ100
Applications
ApplicationThis Anti-CD28 Antibody, clone 15E8 is validated for use in FC, FUNC for the detection of CD28.
Key Applications
  • Flow Cytometry
  • Affects Function
Application NotesTo study the role of CD28 in T cell proliferation. The antibody will induce the proliferation of resting T lymphocytes.

This antibody will act in cell stimulation studies where it has been suggested that measuring T cell function in HIV infected asymptomatic patients may be an early indicator of AIDS, independent of CD4 + cell counts

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman T lymphocytes
Clone15E8
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody reacts with the 44 kDa antigen found on a sub-population of T cells and activated B cells. These CD28+ CD8 cells are now known to be cytotoxic T cell precursors. The antibody does not react with B cells, granulocytes or monocytes. The ligand for CD28 is CD80 (B7) which is found on activated B cells suggesting a role for CD28 in T and B cell interaction

FUSION PARTNER: SP2/0
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryCD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM]
Gene Symbol
  • CD28
  • Tp44
  • TP44
  • MGC138290
Non-Reactive Species
  • Canine
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P10747 # Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.
SIZE: 220 amino acids; 25066 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed in T-cells and plasma cells, but not in less mature B-cells.
SIMILARITY: SwissProt: P10747 ## Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CBL517 04053252589904

Documentation

Anti-CD28 Antibody, clone 15E8 SDS

Title

Safety Data Sheet (SDS) 

Anti-CD28 Antibody, clone 15E8 Certificates of Analysis

TitleLot Number
Anti-CD28, clone 15E8 2484244
Anti-CD28, clone 15E8 - 2398899 2398899
Anti-CD28, clone 15E8 - 2446857 2446857
Anti-CD28, clone 15E8 - 2454700 2454700
Anti-CD28, clone 15E8 - 2463637 2463637
Anti-CD28, clone 15E8 - 2145952 2145952
Anti-CD28, clone 15E8 - 3167053 3167053
Anti-CD28, clone 15E8 - 3187447 3187447
Anti-CD28, clone 15E8 - 3233125 3233125
Anti-CD28, clone 15E8 - 3286048 3286048
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies