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MABF379 Anti-CD127 (IL-7Ra) Antibody (mouse), Biotin, clone A7R34

This rat monoclonal antibody Anti-CD127 (IL-7Ra) (mouse), Biotin, clone A7R34 validated for use in IP, Flow Cytometry & IHC for the detection of CD127 (IL-7Ra).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Il17ra, Il17r UniProt Number: Q60943
MABF379
500 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIP, FC, IHCRBiotinMonoclonal Antibody
Description
Catalogue NumberMABF379
DescriptionAnti-CD127 (IL-7Ra) Antibody (mouse), Biotin, clone A7R34
Alternate Names
  • Interleukin-17 receptor A
  • IL-17 receptor A
  • IL-17RA
  • CD217
Background InformationThe A7R34 antibody is specific for mouse CD127, a 60-90 kDa cell surface protein also known as the Interleukin-7 Receptor alpha chain, or IL-7R alpha. CD127 is typically expressed at the cell surface as a heterodimer with the common gamma chain (CD132). This complex acts as the functional receptor for IL-7, a cytokine important in T and B cell development, and in mature T cell homeostasis. A second cytokine known as Thymic Stromal Lymphopoietin (TSLP) also binds to a receptor complex of CD127 and the TSLPR chain to trigger activation of dendritic cells, and is involved in B cell development, allergy and autoimmunity.The A7R34 antibody may be used as a phenotypic marker for CD127 on immature B cells, on subsets of thymocytes which are double negative (CD4-CD8- ) or single positive (CD4+ or CD8+), and at low levels on mature, peripheral T cells. CD127 is a key marker, when used in combination with CD4 and CD25, to distinguish Treg and effector/memory Treg populations known as T(REM).
Product Information
FormatBiotin
PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis rat monoclonal antibody Anti-CD127 (IL-7Ra) (mouse), Biotin, clone A7R34 validated for use in IP, Flow Cytometry & IHC for the detection of CD127 (IL-7Ra).
Key Applications
  • Immunoprecipitation
  • Flow Cytometry
  • Immunohistochemistry
Biological Information
ImmunogenImmunogen information for this antibody is proprietary information.
CloneA7R34
Concentration0.5 mg/mL
HostRat
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Il17ra
  • Il17r
Purification MethodSize Exclusion Chromatography
UniProt Number
Product Usage Statements
Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.06 µg of this antibody detected CD127 (IL-7Ra) in one million C57Bl/6 splenocytes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Packaging Information
Material Size500 µg
Global Trade Item Number
Catalogue Number GTIN
MABF379 04053252959813