Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABN1669-25UL Anti-Anoctamin-1 (TMEM16A)

Anti-Anoctamin-1 (TMEM16A), Cat. No. ABN1669, is a highly specific rabbit polyclonal antibody that targets Anoctamin-1 (Transmembrane protein 16A) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Research Category: Neuroscience Gene Symbol: Ano1, Tmem16a UniProt Number: Q8BHY3
ABN1669-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, HICC, IF, WBRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABN1669-25UL
DescriptionAnti-Anoctamin-1 (TMEM16A)
Alternate Names
  • Anoctamin-1
  • TMEM16A
Background InformationAnoctamin-1 (UniProt: Q8BHY3; also known as Transmembrane protein 16A, TMEM16A) is encoded by the Ano1 (also known as Tmem16a) gene (Gene ID: 101772) in murine species. Anoctamin-1 is a multi-pass membrane protein that along with TMEMB16B subunit serves as the calcium-activated chloride channels (CaCC). Two isoforms of Anoctamin-1 have been reported that are produced by alternative splicing. It is located in the apical membranes of epithelial cells in exocrine glands and in trachea. Its airway epithelial expression is increased in asthmatics. Hence, its abundance and activity may contribute to mucus secretion and airway hyper-responsiveness in asthma subjects. Anoctamin-1 is reported to be absent from smooth muscle cells, but present in the interstitial cells of Cajal (ICC), the pacemaker cells that control smooth muscle contraction. It acts as a major contributor to basal and stimulated chloride conductance in airway epithelial cells and plays an important role in tracheal cartilage development. ATP and calmodulin are shown to be essential for its activation and the channel activity is inhibited by CFTR protein and by chloride channel inhibitors such as niflumic acid and 4, 4 -diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). Mice with defective Tmem16a gene display severe tracheomalacia with gaps in the tracheal cartilage rings along the entire length of the trachea and over 90% of mutants die within the first nine days of postnatal life.
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-Anoctamin-1 (TMEM16A), Cat. No. ABN1669, is a highly specific rabbit polyclonal antibody that targets Anoctamin-1 (Transmembrane protein 16A) and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected Anoctamin-1 (TMEM16A) in 10 µg of SW-620 and PC3  cell lysates.
Immunofluorescence Analysis: A representative lot detected Anoctamin-1 (TMEM16A) in Immunofluorescence applications (Huang, F., et. al. (2009). Proc Natl Acad Sci USA. 106(50):21413-8; Wang, H.C., et. al. (2015). Cell. 163(6):1348-59; Huang, F., et. al. (2012). Proc Natl Acad Sci USA. 109(40):16354-9).
Immunocytochemistry Analysis: A representative lot detected Anoctamin-1 (TMEM16A) in Immunocytochemistry applications (Huang, F., et. al. (2009). Proc Natl Acad Sci USA. 106(50):21413-8).
Western Blotting Analysis: A representative lot detected Anoctamin-1 (TMEM16A) in Western Blotting applications (Huang, F., et. al. (2009). Proc Natl Acad Sci USA. 106(50):21413-8).
Biological Information
ImmunogenA linear peptide corresponding to 14 amino acids from the N-terminal region of murine Anoctamin-1 (TMEM16A).
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Anoctamin-1/TMEM16A in human and murine cells. it targets an epitope within 14 amino acids from the N-terminal end. May react with both isoforms 1 and 2 based on sequence homology.
IsotypeIgG
Species Reactivity
  • Mouse
  • Human
Species Reactivity NoteHuman, Mouse. Predicted to react with Rat based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Gene Symbol
  • Ano1
  • Tmem16a
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~111 kDa observed; 110.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected Anoctamin-1 (TMEM16A) in 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABN1669-25UL 04054839490101

Documentation