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CBL57 Anti-Adrenocorticotropic Hormone Antibody, NT, clone 57

This Anti-Adrenocorticotropic Hormone Antibody, N-terminus, clone 57 is validated for use in ELISA, IH(P) for the detection of Adrenocorticotropic Hormone.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: CNS Control of Metabolism, Hormones & Receptors Gene Symbol: POMC, pro-ACTH-endorphin, adrenocorticotropin, beta-LPH, POC, gamma-LPH, LPH, ACTH, corticotropin, beta-endorphin, beta-MSH, alpha-MSH, pro-opiomelanocortin, proopiomelanocortin, Pro-opiomelanocortin, corticotropin-lipotrophin, NPP, MSH, met-enkephalin, CLIP, gamma-MSH UniProt Number: P01189
CBL57
1 mg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RELISA, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberCBL57
ReplacesCBL58
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Adrenocorticotropic Hormone Antibody, NT, clone 57
Alternate Names
  • ACTH
References
Product Information
FormatPurified
PresentationThe monoclonal is presented as 1mg in 1ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.
Quality LevelMQ100
Applications
ApplicationThis Anti-Adrenocorticotropic Hormone Antibody, N-terminus, clone 57 is validated for use in ELISA, IH(P) for the detection of Adrenocorticotropic Hormone.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
Application NotesThis antibody reacts with solid phase ACTH either captured as part of a sandwich or adsorbed as a solid phase antigen and as such is suitable for serum assay development

The antibody is also reactive on formalin fixed paraffin wax embedded tissue and is therefore suitable for immunocyto-chemistry

Optimal working dilutions must be determined by the end user.
Biological Information
EpitopeN-terminus
Clone57
HostMouse
SpecificityThis antibody is specific for Synacthen (1-24 ACTH) and has no cross reaction with CLIP (ACTH 17-39). The antibody has an affinity constant greater than 10E11 L/mol.

KNOWN SPECIES CROSS REACTIVITY: Positive with rat N-terminal ACTH peptide
IsotypeIgG1
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described.
Gene Symbol
  • POMC
  • pro-ACTH-endorphin
  • adrenocorticotropin
  • beta-LPH
  • POC
  • gamma-LPH
  • LPH
  • ACTH
  • corticotropin
  • beta-endorphin
  • beta-MSH
  • alpha-MSH
  • pro-opiomelanocortin
  • proopiomelanocortin
  • Pro-opiomelanocortin
  • corticotropin-lipotrophin
  • NPP
  • MSH
  • met-enkephalin
  • CLIP
  • gamma-MSH
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P01189 # Beta-endorphin and Met-enkephalin are endogenous opiates.
SIZE: 267 amino acids; 29424 Da
TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.
PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides. & O-glycosylated; reducing sugar is probably N- acetylgalactosamine.
DISEASE: SwissProt: P01189 # Defects in POMC may be associated with susceptibility to obesity [MIM:601665]. & Defects in POMC are the cause of pro-opiomelanocortinin deficiency [MIM:609734]. Affected individuals present early-onset obesity, adrenal insufficiency and red hair.
SIMILARITY: SwissProt: P01189 ## Belongs to the POMC family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CBL57 04053252275425

Documentation

Anti-Adrenocorticotropic Hormone Antibody, NT, clone 57 SDS

Title

Safety Data Sheet (SDS) 

Anti-Adrenocorticotropic Hormone Antibody, NT, clone 57 Certificates of Analysis

TitleLot Number
MOUSE ANTI-ACTH MONOCLONAL ANTIBODY - 2123660 2123660
MOUSE ANTI-ACTH MONOCLONAL ANTIBODY - 2137006 2137006
MOUSE ANTI-ACTH MONOCLONAL ANTIBODY - 2345001 2345001
MOUSE ANTI-ACTH - 3199249 3199249
MOUSE ANTI-ACTH - 3245102 3245102
MOUSE ANTI-ACTH - 3258714 3258714
MOUSE ANTI-ACTH - 3307745 3307745
MOUSE ANTI-ACTH - 3465943 3465943
MOUSE ANTI-ACTH - 3590108 3590108
MOUSE ANTI-ACTH - 3693109 3693109
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