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MABN2439-25UG Anti-ABCA4 Antibody, clone 3F4

Anti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting.

Anti-ABCA4 Antibody, clone 3F4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: ABCA4 UniProt Number: F1MWM0
MABN2439-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, B, MIHC, IP, IF, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN2439-25UG
DescriptionAnti-ABCA4 Antibody, clone 3F4
Alternate Names
  • ABC transporter A4
  • RIM ABC Transporter
  • RIM protein
Background InformationRetinal-specific ATP-binding cassette transporter (UniProt F1MWM0/P78363; also known as ATP-binding cassette sub-family A member 4, ABC transporter A4, RIM ABC transporter, RIM Protein) is encoded by the ABCA4 gene in bovine species. ABC transporter A4, a ~250 kDa single-chain, is a multi-pass membrane protein of the superfamily of ATP-binding cassette (ABC) transporters that is localized to the disk margins of vertebrate photoreceptor outer segments. It is implicated in the clearance of all-trans retinal derivatives from rod and cone photoreceptor cells following photoexcitation as part of the visual cycle. ABC transporter A4 is composed of two symmetrically organized halves, each comprising six membrane-spanning helices, a large glycosylated exocytoplasmic domain located inside the disk, and a cytoplasmic domain with an ATP-binding cassette. Mutations in ABCA4 are known to cause Stargardt macular degeneration, which is an autosomal recessive form of juvenile macular degeneration associated with a severe loss in vision. (Ref.: Tsybovsky, Y et al. (2010). Adv. Exp. Med. Biol. 703, 105-125).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-ABCA4, clone 3F4, Cat. No. MABN2439, is a highly specific mouse monoclonal antibody that targets ABCA4 (RIM) and has been tested in Immunohistochemistry, Immunoprecipitation, Immunofluorescence, and Western Blotting.
Key Applications
  • Immunohistochemistry
  • Immunoprecipitation
  • Immunofluorescence
  • Western Blotting
Application NotesImmunofluorescence Analysis: A 1:50 dilution from a representative lot detected ABCA4 in mouse retina cells.

Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected ABCA4 in mouse retina cells (Courtesy of Dr. Robert Molday, Ph.D., University of British Columbia, Canada).

Electron Microscopy Analysis: A representative lot detected ABCA4 in Electron Microscopy applications (Illing, M., et. al. (1997). J Biol Chem. 272(15):10303-10).

Immunoprecipitation Analysis: A representative lot detected ABCA4 in Immunoprecipitation applications (Bungert, S., et. al. (2001). J Biol Chem. 276(26):23539-46).

Western Blotting Analysis: A representative lot detected ABCA4 in Western Blotting applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8; Illing, M., et. al. (1997). J Biol Chem. 272(15):10303-10; Beharry, S., et. al. (2004). J Biol Chem. 279(52):53972-9).

Immunohistochemistry Analysis: A representative lot detected ABCA4 in Immunohistochemistry applications (Molday, L.L., et. al. (2000). Nat Genet. 25(3):257-8).
Biological Information
ImmunogenWhole purified bovine disk rim protein.
Clone3F4
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3F4 detects ABC transporter (ABCA4) in Bovine and Human cells. It targets an epitope within 9 amino acids from the C-terminal end.
IsotypeIgG1
Species Reactivity
  • Human
  • Bovine
  • Mouse
Species Reactivity NoteHuman, Bovine, Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • ABCA4
Purification MethodProtein G purified
UniProt Number
Molecular Weight~220 kDa observed; 257.38 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retina tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected ABCA4 in 10 µg of h uman retinal tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN2439-25UG 04054839522246