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ABC1463-25UL Ant-Zyxin

Anti-Zyxin Antibody, Cat. No. ABC1463 is a rabbit polyclonal antibody that detects Zyxin and has been tested for use in Immunocytochemistry and Western Blotting.

Ant-Zyxin MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ZYX UniProt Number: Q15942
ABC1463-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, Ch, HWB, ICCRbSerumPolyclonal Antibody
Description
Catalogue NumberABC1463-25UL
DescriptionAnt-Zyxin
Alternate Names
  • Zyxin-2
Background InformationZyxin (UniProt: Q15942; also known as Zyxin-2) is encoded by the ZYX gene (Gene ID: 7791) in human. Zyxin is an evolutionarily conserved LIM domain protein that acts as an adhesion plaque protein concentrated at sites of cell adhesion and is widely expressed. It has been implicated in cell adhesion, cell motility, and signaling. Zyxin is reported to associate with members of the Enabled (Ena)/vasodilator-stimulated phosphoprotein (VASP) family of cytoskeletal regulators and plays a role in cytoskeletal dynamics and signaling. Zyxin contains three LIM zinc binding domains (aa 384-443; 444-503; and 504-570), which act as a negative regulator of Zyxin-VASP complexes. Also, it contains four proline rich ActA repeats (aa 64-77; 94-108; 115-121; and 127-137) that are known to interact directly with Ena/VASP proteins. Loss of Zyxin results in inability to thicken stress fibers in response to mechanical stimuli. (Ref.: Hoffman LM et al (2003). Mol. Cell Biol. 23(1); 70-79; Moody JD et al. (2009). Biochem. Biophys. Res. Commun. 378(3), 625-628; Yoshigi et al (2005). J. Cell Biol. 171(2), 209-215.).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-Zyxin Antibody, Cat. No. ABC1463 is a rabbit polyclonal antibody that detects Zyxin and has been tested for use in Immunocytochemistry and Western Blotting.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Zyxin in A431 cell lysate.

Immunofluorescence Analysis: A representative lot detected Zyxin in newborn mouse skin. Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2012). Mol Biol Cell. 23(10):1846-59).

Western Blotting Analysis): A representative lot detected Zyxin in mouse lung, mouse embryo fibroblasts and in human Ewing sarcoma cells.(Chaturvedi, A., et. al. (2014). Mol Biol Cell. 25(18):2695-709; Hoffman, L.M., et. al. (2003). Mol Cell Biol. 23(1):70-9; Hoffman, L.M., et. al. (2012). Mol Biol Cell. 23(10):1846-59; Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82).

Immunocytochemistry Analysis: A representative lot detected Zyxin at focal adhesions in mouse fibroblasts and in 4T1 cells with ATG7 shRNA or ATG5 shRNA (Hoffman, L.M., et. al. (2006). J Cell Biol. 172(5):771-82; Sharifi, M.N., et. al. (2016). Cell Rep. 15(8):1660-72).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal half of human Zyxin.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Zyxin in human, mouse, and chicken cells. It targets an epitope within 20 amino acids from the C-terminal half.
IsotypeIgG
Species Reactivity
  • Mouse
  • Chicken
  • Human
Species Reactivity NoteHuman, Mouse, Chicken.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ZYX
Purification MethodUnpurified
UniProt Number
Molecular Weight~75 kDa observed; 61.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Zyxin in NIH3T3 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABC1463-25UL 04054839468902