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176871 Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Peptidyl-Dipetidase A, ACE

176871
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Overview

Products

Catalogue NumberPackaging Qty/Pack
176871-10UG Plastic ampoule 10 μg
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Description
OverviewRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
Catalogue Number176871
Brand Family Calbiochem®
SynonymsPeptidyl-Dipetidase A, ACE
References
ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
FormLiquid
FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
Quality LevelMQ100
Biological Information
Purity≥95% by SDS-PAGE
Specific Activity≥1 unit/µg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
ContaminantsEndotoxins: ≤1.0 EU/µg ACE
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
176871-10UG 04055977222982