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SCR104 Accutase®XL Lyophilized Accutase

AccutaseXL is a lyophilized form of Accutase with extended stability used for the routine detachment of cells from standard tissue culture plasticware

Accutase®XL Lyophilized Accutase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Harvesting, Cell Culture
SCR104
500ml  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Key ApplicationsSource
Harv, CULTAccutase is derived from a non-mammalian (invertebrate) source.
Description
Catalogue NumberSCR104
Brand Family Chemicon®
DescriptionAccutase®XL Lyophilized Accutase
Background InformationAccutase is a cell detachment solution of proteolytic and collagenolytic enzymes that is useful for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware, including SmartPlastic™. Accutase is direct replacement for trypsin cell detachment solution and does not require enzyme inactivation with serum. Accutase does not contain mammalian or bacterial derived products.

AccutaseXL is a lyophilized form of Accutase with extended stability. AccutaseXL, once reconstituted, will make 500ml of complete 1X Accutase solution. Note: read detailed reconstitution protocol found in the product datasheet before using this product.
References
Product Information
Presentation1X Accutase enzymes in Dulbecco’s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4) containing 0.5 mM EDTA4Na and 3 mg/L Phenol Red.
Quality LevelMQ300
Applications
ApplicationAccutaseXL is a lyophilized form of Accutase with extended stability used for the routine detachment of cells from standard tissue culture plasticware
Key Applications
  • Cell Harvesting
  • Cell Culture
Application NotesAccutase has been shown to be effective on a wide variety of cell types including: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.
Biological Information
SourceAccutase is derived from a non-mammalian (invertebrate) source.
Media FormLiquid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancepH: 6.8-7.8
Activity Assay: 500-20 units/ml (1 unit will release 1 µmole pNA from SUCR-pNA per minute at 37˚C)
Functional Assay: Cell detachment assay
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore lyophilized AccutaseXL at -20°C for up to 4 months from date of receipt.
Once reconstituted in water, liquid Accutase can be aliquoted and frozen at -20°C for 2 years or stored for 2 months at 4°C. It is recommended to thaw liquid Accutase at 4°C overnight or in a bath of cool water.
Packaging Information
Material Size500ml
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCR104 04053252992872