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100497 AFB-Color modified

Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method

AFB-Color modified MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
100497
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
1004970001 Fibre carton 1 unit
Description
Catalogue Number100497
DescriptionAFB-Color modified
OverviewThe AFB-Color modified - Staining kit for the detection of acid-fast bacteria (AFB) by hot staining method, is used for human-medical cell diagnosis and serves the purpose of the bacteriological investigation of sample material of human origin, for example smears of enriched bacterial cultures.
AFB-Color modified staining set uses the classical Ziehl-Neelsen hot staining procedure and can be used for staining on the staining rack. The cell wall of acid-fast bacteria has a high proportion of wax and lipids and hence absorbs dyes only very slowly. The most efficient staining method is the hot staining according to Ziehl-Neelsen. In this method, carbolfuchsin solution is applied to the specimen and heated. This heating process accelerates the rate at which the fuchsin dye is absorbed and thus the formation of the mycolate-fuchsin complex in the cell wall. Once the acid-fast bacteria have absorbed the fuchsin dye, it is virtually impossible to decolorize them again, even when they are intensively treated with a decolorizing solution such as e. g. hydrochloric acid in ethanol. Accordingly, acid-fast bacteria are termed as acid- and alcohol-fast for staining, and are stained red in the microscopic visualization. Correspondingly, all non-acid-fast microorganisms are counterstained with an appropriate dye. This AFB-Color modified staining kit uses methylene blue as counterstain.
Pretreatment of the specimens with Sputofluol® dissolves the bacteria from the surrounding viscid sputum and cell material.
The package is sufficient for approx. 250 applications. This product is registered as IVD and CE marked.For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
References
Product Information
HS Code3212 90 00
Quality LevelMQ400
Applications
ApplicationStaining kit for the detection of acid-fast bacteria (AFB) by hot staining method
Biological Information
Physicochemical Information
Flash point21 °C
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Hazard Pictogram(s) Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Pictogram(s)
Hazard Statement(s)H225: Highly flammable liquid and vapour.
H290: May be corrosive to metals.
H314: Causes severe skin burns and eye damage.
H341: Suspected of causing genetic defects.
H410: Very toxic to aquatic life with long lasting effects.
Precautionary Statement(s)P210: Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
P273: Avoid release to the environment.
P280: Wear protective gloves/ protective clothing/ eye protection/ face protection/ hearing protection.
P303 + P361 + P353: IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water.
P304 + P340 + P310: IF INHALED: Remove person to fresh air and keep comfortable for breathing. Immediately call a POISON CENTER/doctor.
P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P403 + P233: Store in a well-ventilated place. Keep container tightly closed.
Signal WordDanger
Storage class3 Flammable liquids
Safety Information
Product Usage Statements
Storage and Shipping Information
StorageStore at +15°C to +25°C.
Packaging Information
Transport Information
Declaration (railroad and road) ADR, RIDUN 2924 Entzündbarer flüssiger Stoff, ätzend, n.a.g.(SALZSAEURE UND ENTH. ETHANOL), 3 (8), II
Declaration (transport by air) IATA-DGRUN 2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S.(HYDROCHLORIC ACID AND CONT. ETHANOL), 3 (8), II
Declaration (transport by sea) IMDG-CodeUN 2924 FLAMMABLE LIQUID, CORROSIVE, N.O.S.(HYDROCHLORIC ACID AND CONT. ETHANOL), 3 (8), II, Segregation Group: 1 (Acids)
Supplemental Information
Specifications
Suitability for microscopypasses test
Mycobacterium tuberculosisred
Backgroundblue
Global Trade Item Number
Catalogue Number GTIN
1004970001 04022536703619

Related Products & Applications

Alternative Products

Catalogue Number Description
116450 AFB-Color staining kit

Related Products

Catalogue Number Description
109215 Ziehl-Neelsen carbol-fuchsin solution
116450 AFB-Color staining kit

Accessories (Primary)

Catalogue Number Description
100327 Hydrochloric acid in ethanol
101287 Löffler's methylene blue solution
109215 Ziehl-Neelsen carbol-fuchsin solution

Categories

IVD/OEM Materials & Reagents > Microscopy > Histology > Histological Staining Kits
IVD/OEM Materials & Reagents > Microscopy > Bacteriology > Acid-Fast Bacteria Staining