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SCM304 3dGRO™ Human Colon Organoid Expansion Medium

Complete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.

SCM304
50 ml  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberSCM304
Description3dGRO™ Human Colon Organoid Expansion Medium
Background Information3dGRO™ Human Colon Organoid Expansion Medium is a complete ready-to-use serum-free medium for the expansion and long-term culture of human colonic organoids. The medium has been validated for use with 3dGRO™ Human iPSC Derived Colon Organoids (Cat. No. SCC300). Colon organoids propagated in the medium express colon-specific markers including the posterior hindgut marker CDX2, a-carbonic anydrase II (CA-II), a-carbonic anhydrase IV (CA-IV), and goblet cell markers Mucin-2 and Mucin-5B.
References
Product Information
Applications
ApplicationComplete serum-free organoid media for the expansion and long-term 3D culture of human intestinal colon organoids.
Key Applications
  • Cell Culture
  • Stem Cell Culture
Biological Information
Media FormLiquid
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance• Appearance (Color): Clear/No Particlulates (Red Liquid)
• Osmolality: 350-375 mOsm
• pH: 7.0 – 7.4
• Sterility Tested: No Growth/Pass
• Endotoxin: <2 EU/mL
• Mycoplasma: Negative
• Functional Assay: Thaw and culture of human colon organoids for 2 passages.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store at -20°C. When ready to use, thaw overnight at 2-8°C. Once thawed, mix thoroughly. Use immediately and store at 2-8°C for up to 1 week. Do not re-freeze. Unused aliquots may be stored at -20°C until the expiry date.
Packaging Information
Material Size50 ml
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
SCM304 04054839986451