| CellASIC® ONIX Microfluidic Platform (EV262 Microfluidic System & MIC230 Microincubator Controller) | |
|---|---|
| Microscope Compatibility | Inverted microscope |
| Microscopy Techniques | Fluorescence, Brightfield, Phase Contrast, TIRF, Confocal, and DIC Microscopy |
| Analysis Substrate | #1.5 glass coverslip |
| Microfluidic Plate Footprint | 96-well plate footprint |
| Number of Chambers | 4 microfluidic cell culture chambers (in parallel) |
| Culture Time with CellASIC® ONIX Microfluidic Controller | 1-3 days continuous |
| Cell Suspension Volume | 5-10 µL (M04 CellASIC® ONIX Microfluidic Plates) 50 µl (B04/Y04/C04 CellASIC® ONIX Microfluidic Plates) |
| Number of Pressure Inputs | 8 inputs |
| Output Pressure Range | 0-10±0.25 psi (0-70±1.7 kPa) |
| Optical Transparency | Optically clear manifold and microfluidic plates |
| Optional Premixed Gas Input | Works with clean, dry, premixed gas containing air, CO2, nitrogen and oxygen up to 25% regulated to between 45-55 psi (310-379 kPa). |
| Temperature Control Range | Room temp. to 40 °C |
| Rise Time (25 °C to 37 °C) | <10 minutes |
| Cooling Time (37 °C to 25 °C) | <15 minutes |
| Gas Consumption | 3 mL/min, ±0.5 mL/min |
| Dimensions | 310 mm Wide x 257 mm Deep x 163 mm Height |
CellASIC® ONIX Microfluidic Platform Specifications |
Request Information |
| General | |
|---|---|
| Dimensions | System: 330 mm wide × 306 mm deep × 108 mm high (13 in. × 12 in. × 4.25 in.) |
| Weight | System: 6.7 kg (14.75 lb) |
| Power consumption | 100–240 VAC, 50/60 Hz 40 W |
| Cooling mode | Air cooled (natural convection) |
| Environmental conditions | For indoor laboratory use |
| Operating temperature | 20 °C to 30 °C |
| Storage temperature | 5 °C to 35 °C |
| Flow Control | |
|---|---|
| Number of outputs | 8 (each addressable by either of two pressure controllers) |
| Pressure range | -50 to 70 kPa (-7.25–10.2 psi) |
| Pressure accuracy | ± 1.5 kPa (0.22 psi) |
| Pressure stabilization time | within ± 5 kPa (0.73 psi) in < 5 seconds |
| Gas Environment Control | |
|---|---|
| Control capabilities | Option to electronically select between two premixed gasses and set flow rate to slow or fast. |
| Gas input requirements | Clean, dry, premixed gas mixtures containing air, CO2, N2, and oxygen (up to 25%), regulated to between 100 kPa and 700 kPa (15 psi and 100 psi) |
| Gas consumption | Slow flow: 5 mL/min ± 2 mL/min Fast flow: 50 mL/min ± 20 mL/min |
| Cell culture region gas environment accuracy | For gas flow at 3 mL/min: < 10% deviation from delivered gas concentration For gas flow at 30 mL/min: < 2% deviation from delivered gas concentration |
| Temperature Control | (Requires CAX2-MXT20 CellASIC ONIX2 Manifold XT) |
|---|---|
| Temperature control range | Room temperature to 40 °C |
| Control method | Bi-directional PID |
| Accuracy of sample chamber temperature | ± 1 °C (using the CAX2-ACT20 ONIX2 Temperature Calibration Plate can result in accuracy as high as ± 0.2 °C) |
| Stability of sample chamber temperature | ± 0.2 °C |
| Rise time (25 °C to 37 °C) | < 30 minutes |
| Cooling time (37 °C to 25 °C) | < 30 minutes |
| Computer Requirements | |
|---|---|
| Operating system | Windows® 7, or Windows® 10 (32 or 64 bit) |
| Random access memory (RAM) | 4 GB or higher |
| Monitor | 1920x1080 resolution or higher |
| Hard drive | 200 MB or higher |
| Hardware interface | USB 2.0 or higher |
| Microscope Requirements | |
|---|---|
| Type | Inverted microscope |
| Stage weight capacity | For CAX2-MXT20 CellASIC ONIX2 Manifold XT and filled plate: 325 g (11.1 oz) For CAX2-MBC20 CellASIC ONIX2 Manifold Basic and filled plate: 150 g (5.3 oz) |
| Minimum condenser working distance | For CAX2-MXT20 CellASIC ONIX2 Manifold XT: 28 mm (0.9 in) For CAX2-MBC20 CellASIC ONIX2 Manifold Basic: 28 mm (0.9 in) |
| Maximum condenser diameter | For CAX2-MXT20 CellASIC ONIX2 Manifold XT: 70 mm (2.8 in) For CAX2-MBC20 CellASIC ONIX2 Manifold Basic: no limit |