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71110 rLysozyme™ Solution

Stabilized recombinant lysozyme

rLysozyme™ Solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71110
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71110-3
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      71110-4
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          71110-5
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              Description
              OverviewrLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
              Catalogue Number71110
              Brand Family Novagen®
              Product Information
              Unit of DefinitionOne unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.
              Quality LevelMQ400
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Standard Handling
              Storage -20°C
              Avoid freeze/thaw Avoid freeze/thaw
              Do not freeze Ok to freeze
              Global Trade Item Number
              Catalogue Number GTIN
              71110-3 07790788055547
              71110-4 04055977254617
              71110-5 07790788053048