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23-024 p27 Protein complex, activated, 10 µg

Activated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ub

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p27 Protein complex, activated, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Enzyme Assays
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23-024
10 µg  
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      Overview

      Key Spec Table

      Key ApplicationsSpeciesCascade Component
      Enzyme AssaysHuman Substrate
      Description
      Catalogue Number23-024
      Brand Family Upstate
      Trade Name
      • Upstate
      Descriptionp27 Protein complex, activated, 10 µg
      OverviewC-terminal cMyc and 6His-tagged, recombinant human Smac/DIABLO amino acids 56-end, expressed in E.coli
      Alternate Names
      • p27kip1
      • Cyclin-dependent kinase inhibitor 1B
      • Kip1
      Background Informationp27 is an important regulator of cell cycle progression, involved in G1 arrest. It is a potent inhibitor of cyclin E1- and cyclin A-CDK2 complexes and a positive regulator of cyclin D-dependent kinases such as CDK4. The degradation of this protein, which is triggered by its CDK-dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Decreased levels of p27 are found in various epithelial tumours originating from lung, breast, colon, ovary, oesophagus, thyroid and prostate. Defects in the gene encoding p27 (CDKN1B) are the cause of multiple endocrine neoplasia type 4 (MEN4) an inherited cancer of the thyroid.
      Product Information
      Quality LevelMQ100
      Applications
      ApplicationActivated complex of three (3) full length, recombinant human proteins: p27, Cyclin E1, & CDK2. Phosphorylated in vitro using CDK2/Cyclin E1. For use in Enzyme Assays. Behaves as a substrate in ubiquitin assays.
      Key Applications
      • Enzyme Assays
      Biological Information
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Cascade ComponentSubstrate
      Entrez Gene Number
      Purification MethodPurified using immobilized metal affinity chromatography.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Packaging Information
      Material Size10 µg
      Global Trade Item Number
      Catalogue Number GTIN
      23-024 04053252294693