Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
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-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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506300
Sigma-AldrichTrkB Agonist, 7,8-Dihydroxyflavone - CAS 38183-03-8 - Calbiochem
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Description
Overview
A cell permeable flavone based compound with neurotrophic and neuroprotective properties. Mimicks the action of Brain Derived Neurotrophic Factor (BDNF) and acts as a TrkB receptor agonist. Due to its lipophilic nature, it can cross the blood-brain barrier. Binds to the extra cellular domain of TrkB with high affinity and promotes its dimerization and autophosphorylation. Potentiates TrkB, but not TrkA, signaling in mouse brain slices. Its neurotropic effects are abolished in the presence of K252a. Suppresses apoptosis in human and rodent neurons and in stably transfected TrkB murine cell line T48 (EC50 = 35 nM). Selectively reduces the basal GABAergic transmission due to its suppressive effect on presynaptic GABA release. However, it does not affect glutamatergic transmission.
Catalogue Number
506300
Brand Family
Calbiochem®
Synonyms
7,8-DHF
References
References
Ren, Q., et al. 2013. Pharm. Biochem. Behav.106, 124. Marongiu, D., et al. 2013. Eur. J. Pharmacol.709, 64. Jang, S., et al. 2010. PNAS107, 2687.
Ren, Q., et al. 2013. Pharm. Biochem. Behav.106, 124. Marongiu, D., et al. 2013. Eur. J. Pharmacol.709, 64. Jang, S., et al. 2010. PNAS107, 2687.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
22-November-2013 JSW
Synonyms
7,8-DHF
Description
A cell permeable flavone based compound with neurotrophic and neuroprotective properties. Mimicks the action of Brain Derived Neurotrophic Factor (BDNF) and acts as a TrkB receptor agonist. Due to its lipophilic nature, it can cross the blood-brain barrier. Binds to the extra cellular domain of TrkB with high affinity and promotes its dimerization and autophosphorylation. Potentiates TrkB, but not TrkA, signaling in mouse brain slices. Its neurotropic effects are abolished in the presence of K252a. Suppresses apoptosis in human and rodent neurons and in stably transfected TrkB murine cell line T48 (EC50 = 35 nM). Selectively reduces the basal GABAergic transmission due to its suppressive effect on presynaptic GABA release. However, it does not affect glutamatergic transmission.
Form
Yellow powder
Intert gas (Yes/No)
Packaged under inert gas
CAS number
38183-03-8
Chemical formula
C₁₅H₁₀O₄
Structure formula
Purity
≥98% by HPLC
Solubility
DMSO (100 mg/ml)
Storage
Protect from light +2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity
Standard Handling
References
Ren, Q., et al. 2013. Pharm. Biochem. Behav.106, 124. Marongiu, D., et al. 2013. Eur. J. Pharmacol.709, 64. Jang, S., et al. 2010. PNAS107, 2687.
