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595501 ThioGlo® 1 Fluorescent Thiol Reagent

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3H-Naphthol[2,1-b]pyran-s-carboxylic Acid, 10-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-, Methyl Ester

595501
  
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Purity
      137350-66-4≥99% by TLC
      Description
      Overview

      This product has been discontinued.



      We are offering Thiol Fluorescent Probe IV (Cat. No. 595504)as a possible alternative. Please read the alternative product documentation carefully and contact technical service if you need additional information.






      Maleimide reagent for the detection, determination, and derivatization of thiol groups using fluorescent techniques. Superior to other thiol modifying reagents due to high fluorescent quantum yields after reaction with SH (QYSH = 0.49), extremely low fluorescence from unreacted ThioGlo® Reagent (QYR = 0.008), fast reaction times (5000 times faster than bimanes), resistance to hydrolysis (t½ ≥8.5 hr at 25°C, pH 7.0), non-interfering hydrolysis products, minimal photobleaching, large Stokes shifts (134 nm), solvachromic properties, and high sensitivity (QYSH/QYR = 60).
      Catalogue Number595501
      Brand Family Calbiochem®
      Synonyms3H-Naphthol[2,1-b]pyran-s-carboxylic Acid, 10-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-, Methyl Ester
      References
      ReferencesStorey, B.T., et al. 1998. Mol. Reprod. Dev. 49, 400.
      Wright, S.K. and Viola, R.E. 1998. Anal. Biochem. 265, 8.
      Langmuir, M.E., et al. 1996. in Fluorescence Microscopy and Fluorescent Probes (Slavic, J., ed.) pp. 229-233, Plenum Press, New York.
      Product Information
      CAS number137350-66-4
      FormCrystalline solid
      Hill FormulaC₂₀H₁₃NO₇
      Chemical formulaC₂₀H₁₃NO₇
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Purity≥99% by TLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      595501 0

      Documentation

      ThioGlo® 1 Fluorescent Thiol Reagent Certificates of Analysis

      TitleLot Number
      595501

      References

      Reference overview
      Storey, B.T., et al. 1998. Mol. Reprod. Dev. 49, 400.
      Wright, S.K. and Viola, R.E. 1998. Anal. Biochem. 265, 8.
      Langmuir, M.E., et al. 1996. in Fluorescence Microscopy and Fluorescent Probes (Slavic, J., ed.) pp. 229-233, Plenum Press, New York.

      Citations

      Title
    • Yusheng Han, et al. (2005) Ethacrynic Acid Inhibits Multiple Steps in the NF-κB Signaling Pathway. Shock 23, 45-53.
    • John R. Pawloski, Douglas T. Hess and Jonathan S. Stamler. (2005) Impaired vasodilation by red blood cells in sickle cell disease. Proceedings of the National Academy of Science USA 102, 2531-2536.
    • Joshua D. Nosanchuk, et al. (2003) Antibodies to a cell surface histone-like protein protect against Histoplasma capsulatum. Journal of Clinical Investigation 112, 1164-1175.
    • Huixing Wu, et al. (2003) Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability. Journal of Clinical Investigation 111, 1589-1602.
    • P. J. Britto, Leslie Knipling and J. Wolff. (2002) The Local Electrostatic Environment Determines Cysteine Reactivity of Tubulin. Journal of Biological Chemistry 277, 29018-29027.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-April-2011 RFH
      Synonyms3H-Naphthol[2,1-b]pyran-s-carboxylic Acid, 10-(2,5-Dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-, Methyl Ester
      DescriptionMaleimide reagent for the detection, determination, and derivatization of thiol groups using fluorescent techniques. Superior to other thiol modifying reagents due to high fluorescent quantum yields after reaction with SH (QYSH = 0.49), extremely low fluorescence from unreacted ThioGlo® Reagent (QYR = 0.008), fast reaction times (5000 times faster than bimanes), resistance to hydrolysis (t½ ≥8.5 hr at 25°C, pH 7.0), non-interfering hydrolysis products, minimal photobleaching, large Stokes shifts (134 nm), solvachromic properties, and high sensitivity (QYSH/QYR = 60).
      FormCrystalline solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number137350-66-4
      Chemical formulaC₂₀H₁₃NO₇
      Structure formulaStructure formula
      Purity≥99% by TLC
      SolubilityDMF or DMSO (5 mg/ml)
      Storage Protect from moisture
      Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesStorey, B.T., et al. 1998. Mol. Reprod. Dev. 49, 400.
      Wright, S.K. and Viola, R.E. 1998. Anal. Biochem. 265, 8.
      Langmuir, M.E., et al. 1996. in Fluorescence Microscopy and Fluorescent Probes (Slavic, J., ed.) pp. 229-233, Plenum Press, New York.
      Citation
    • Yusheng Han, et al. (2005) Ethacrynic Acid Inhibits Multiple Steps in the NF-κB Signaling Pathway. Shock 23, 45-53.
    • John R. Pawloski, Douglas T. Hess and Jonathan S. Stamler. (2005) Impaired vasodilation by red blood cells in sickle cell disease. Proceedings of the National Academy of Science USA 102, 2531-2536.
    • Joshua D. Nosanchuk, et al. (2003) Antibodies to a cell surface histone-like protein protect against Histoplasma capsulatum. Journal of Clinical Investigation 112, 1164-1175.
    • Huixing Wu, et al. (2003) Surfactant proteins A and D inhibit the growth of Gram-negative bacteria by increasing membrane permeability. Journal of Clinical Investigation 111, 1589-1602.
    • P. J. Britto, Leslie Knipling and J. Wolff. (2002) The Local Electrostatic Environment Determines Cysteine Reactivity of Tubulin. Journal of Biological Chemistry 277, 29018-29027.
      		•